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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

MG-63 (human, bone, osteosarcoma)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Passages: 107; Karyotype: Modal No. 66; hypotriploid
  From osteosarcoma of 14 year male. Produces high yields of interferon following superinduction with polyinosinic acid-polycytidilic acid, cyclohexamide and actinomycin. Antigenically, MG-63 interferon is more closely related to human fibroblast than to leukocyte interferon.
  Research council deposit: No; Price Code: A;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CRL 1427, IZSBS: BS TCL40, ICLC: HTL99003

Bibliographic references:

• Antimicrob Agents Chemother 1977;12:11-15 - PMID: 883813
  

MG-63 (human, bone, osteosarcoma)

Available in the following LABORATORY:

• Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
  MEM (EBSS) + 10% inactivated FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 96 , mycoplasma negative, culture
  Producing high yields of interferon; antigenically MG63 interferon is said to be more closely related to human fibroblast than to leukocyte interferon.
  

Availability in cell line catalogues: ATCC: CRL 1427, ECACC: 86051601, ICLC: HTL99003

MG-63 (human, bone, osteosarcoma)

Available in the following LABORATORY:

• Centro di Riferimento Oncologico (PNIOS, Aviano)
  DMEM + 10% FBS; Freezing medium: DMEM + 32% FBS + 8% DMSO; 37C, 5% CO2, split 1-5
  

Availability in cell line catalogues: ATCC: CRL 1427, IZSBS: BS TCL40, ECACC: 86051601, ICLC: HTL99003

Bibliographic references:

• Antimicrob Agents Chemother 1977;12:11-15 - PMID: 883813

MG-63 (human, bone, osteosarcoma)

Available in the following LABORATORY:

• Russian Academy of Sciences (SPBIC, St. Petersburg)
  
  

Availability in cell line catalogues: ATCC: CRL 1427, IZSBS: BS TCL40, ECACC: 86051601, ICLC: HTL99003

Bibliographic references:

• Antimicrob Agents Chemother 1977;12:11-15 - PMID: 883813