J774A.1's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

J774A.1 (mouse, BALB/c, blood, not defined tumor)

SPECIES: mouse, BALB/c
TISSUE/ORGAN: blood
TUMOR: not defined tumor

ECACC catalog code: 91051511
Morphology: Macrophage
Description: Mouse BALB/c monocyte macrophage
Viruses: See Properties
Depositor: Not registered
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Immunoglobulin, complement Products: Interleukin 1 (IL-1), lysozone Applications: Metabolic studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 3-9x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 9; Derived from a tumour in a female BALB/c mouse. Cell line produces IL-5 and lysozyme.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: TIB 67, ICLC: ATL98011

Bibliographic references:

Cancer Res 1977;37:546-550 - PMID: 318922
J Exp Med 1976;143:1528-1533 - PMID: 1083890
J Immunol 1975;114:898-905 - PMID: 1089721
J Immunol 1977;119:950-954 - PMID: 894031
Nature 1975;257:393-394 - PMID: 1101071

J774A.1 (mouse, BALB/c, monocyte-macrophage, not defined tumor)

Monoclonal continuous culture, grown as monolayer
macrophage morphology
SPECIES: mouse, BALB/c (female)
TISSUE/ORGAN: monocyte-macrophage
TUMOR: not defined tumor
PROPERTIES: IL-1 prod.; lysozyme synthesis; complement receptor; Fc receptor; biochemistry; metabolism; Ig and complement receptor

Available in the following LABORATORY:

Pharmacia Upjhon (MIECC, Nerviano (MI))
DMEM + 10% FBS + 1% non-essential amino acids + NaHCO3 (24mM) + Tricine (20mM); Freezing medium: DMEM + 20% FBS + 10% Glycerol; 37C, 5% CO2, split 1-3 , mycoplasma negative

Availability in cell line catalogues: ATCC: TIB 67, ECACC: 91051511, ICLC: ATL98011

Bibliographic references:

Cancer Res 1977;37:546-550 - PMID: 318922
J Exp Med 1976;143:1528-1533 - PMID: 1083890
J Immunol 1975;114:898-905 - PMID: 1089721
J Immunol 1977;119:950-954 - PMID: 894031
Nature 1975;257:393-394 - PMID: 1101071

J774A.1 (mouse, BALB/c, monocyte-macrophage, not defined tumor)

Continuous culture, grown as monolayer
macrophage morphology
SPECIES: mouse, BALB/c (female)
TISSUE/ORGAN: monocyte-macrophage
TUMOR: not defined tumor
PROPERTIES: IL-1 prod.; lysozyme synthesis; complement receptor; Fc receptor; biochemistry; metabolism; Ig and complement receptor

Available in the following LABORATORY:

Centro di Riferimento Oncologico (PNIOS, Aviano)
DMEM + 10% FBS; Freezing medium: DMEM + 32% FBS + 8% DMSO; 37C, 5% CO2, split 1-5

Availability in cell line catalogues: ATCC: TIB 67, ECACC: 91051511, ICLC: ATL98011

Bibliographic references:

Cancer Res 1977;37:546-550 - PMID: 318922
J Exp Med 1976;143:1528-1533 - PMID: 1083890
J Immunol 1975;114:898-905 - PMID: 1089721
J Immunol 1977;119:950-954 - PMID: 894031
Nature 1975;257:393-394 - PMID: 1101071

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105