ZR-75-1's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

ZR-75-1 (human, Caucasian, breast, carcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: breast
TUMOR: carcinoma

ECACC catalog code: 87012601
Morphology: Epithelial
Description: Human Caucasian breast carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Reported to express the oestrogen and progesterone receptors Applications: Hormone and tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 1mM Nap + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 97; Karyotype: 2n = 46, modal No 72
Derived from a malignant ascitic effusion in a 63 year old female Caucasian with infiltrating ductal carcinoma. The cells are reported to express both the wildtype and variant oestrogen receptors, progesterone receptor and other steroid hormones.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1500, IZSBS: BS TCL75, ICLC: HTL99006

Bibliographic references:

Cancer Res 1978;38:3352-3364 - PMID: 688225
Cancer Res 1978;38:4327-4339 - PMID: 212193

ZR-75-1 (human, Caucasian, breast, carcinoma, ductal)

ICLC catalog code: HTL99006
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 63 years old)
TISSUE/ORGAN: breast
TUMOR: carcinoma, ductal
DEPOSITOR: Prof. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: positive for estrogen receptor (ER+)

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)

Availability in cell line catalogues: IZSBS: BS TCL75, ECACC: 87012601

Bibliographic references:

Cancer Res 1978;38:3352-3364 - PMID: 688225
Cancer Res 1978;38:4327-4339 - PMID: 212193
Int J Food Sci Nutr 2000;51:S95-103 - PMID: 11271861

ZR-75-1 (human, Caucasian, breast, carcinoma)

IZSBS catalog code: BS TCL75
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 63 years old)
TISSUE/ORGAN: breast
TUMOR: carcinoma
PROPERTIES: specialized product secr.; chemosensibility; receptor; chemotherapeutic agents; hormone sensibility; tumorigenicity; cell biology; steroid hormones sensitive; estrogen receptor positive

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Morphology: flat.

Availability in cell line catalogues: ATCC: CRL 1500, ECACC: 87012601, ICLC: HTL99006

ZR-75-1 (human, Caucasian, breast, carcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 63 years old)
TISSUE/ORGAN: breast
TUMOR: carcinoma
PROPERTIES: specialized product secr.; chemosensibility; receptor; chemotherapeutic agents; hormone sensibility; tumorigenicity; cell biology; steroid hormones sensitive; estrogen receptor positive

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)
mycoplasma negative
Receptors for estrogen and other steroid hormones.

Availability in cell line catalogues: ATCC: CRL 1500, IZSBS: BS TCL75, ECACC: 87012601, ICLC: HTL99006

Bibliographic references:

Cancer Res 1978;38:3352-3364 - PMID: 688225
Cancer Res 1978;38:4327-4339 - PMID: 212193

ZR-75-1 (human, Caucasian, breast, carcinoma)

Available in the following LABORATORY:

Dip. Scienze Biomediche ed Oncologia Umana (TOUCI, Torino)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 5% or 10% DMSO; 37C, 5% CO2, split 1-4 Passage: 100

Availability in cell line catalogues: ATCC: CRL 1500, IZSBS: BS TCL75, ECACC: 87012601, ICLC: HTL99006

Bibliographic references:

Cancer Res 1978;38:3352-3364 - PMID: 688225
Cancer Res 1978;38:4327-4339 - PMID: 212193

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105