YAC-1's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

YAC-1 (mouse, A/Sn, lymphoma)

Continuous culture, grown as suspension
lymphoblast morphology
SPECIES: mouse, A/Sn (newborn)
TUMOR: lymphoma
TRANSFORMED BY: 55
PROPERTIES: cytotoxicity; antitumour testing; natural killer assay

Available in the following LABORATORY:

Istituto Nazionale Riposo e Cura Anziani (INRCA) (ANIIR, Ancona)
RPMI 1640 + 10% FBS; Freezing medium: 90% FBS + 10% DMSO

Availability in cell line catalogues: ATCC: TIB 160, ECACC: 86022801, DSMZ: ACC 96

Bibliographic references:

Eur J Immunol 1975;5:112 - PMID: 1234049

SPECIES: mouse, A/Sn
TUMOR: lymphoma

ECACC catalog code: 86022801
Morphology: Lymphoblast
TRANSFORMED BY: Moloney-MuLV
Description: Mouse lymphoma
Viruses: See Properties
Depositor: Prof H Harris/Dr C Madin, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: NK assays

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Induced by inoculation of Moloney leukaemia virus into a new-born A/Sn mouse. Sensitive to the cytotoxic activity of naturally occurring killer cells in mice (NK cells). Often used as target cells in NK assays.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: TIB 160, DSMZ: ACC 96

Bibliographic references:

Eur J Immunol 1975;5:112 - PMID: 1234049

YAC-1 (mouse, A/Sn, lymphoma)

Continuous culture, grown as suspension
lymphoblast morphology
SPECIES: mouse, A/Sn (newborn)
TUMOR: lymphoma
TRANSFORMED BY: 55
PROPERTIES: cytotoxicity; antitumour testing; natural killer assay

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Lymphoma induced in vivo by Moloney leukemia virus

Availability in cell line catalogues: ATCC: TIB 160, ECACC: 86022801, DSMZ: ACC 96

Bibliographic references:

Eur J Immunol 1975;5:112 - PMID: 1234049

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105