Continuous culture, grown as suspension
lymphoblast morphology
SPECIES: mouse, A/Sn (newborn)
TUMOR: lymphoma
TRANSFORMED BY: 55
PROPERTIES: cytotoxicity; antitumour testing; natural killer assay
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: TIB 160, ECACC: 86022801, DSMZ: ACC 96
Bibliographic references:
- Eur J Immunol 1975;5:112 - PMID: 1234049
SPECIES: mouse, A/Sn
TUMOR: lymphoma
DSMZ catalog code: ACC 96
Morphology: round to oval, single or clustered cells in suspension
TRANSFORMED BY: Moloney-MuLV
Description: mouse lymphoma established by inoculation of the Moloney leukemia virus (MLV) into a newborn A/Sn mouse; cells were described to be sensitive to the cytotoxic activity of natural killer (NK) cells and are therefore often used as target cells in NK assays confirmed as mouse with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Prof. P. F. Muehlradt, GBF, Braunschweig, Germany
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.3-2.0 x 106 cells/ml; cells grow very fast; split ratio of 1:5 to 1:10; seed out at ca. 5 x 105 cells/ml at 37C with 5% CO2 maximal density of about 3.0 x 106 cells/ml;
doubling time of ca. 20 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hyperdiploid, 8% polyploid; 41(38-42)<2n>; mouse chromosome morphology confirmed) * isoenzymes (confirmed as mouse with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: TIB 160, ECACC: 86022801
Bibliographic references:
- Eur J Immunol 1975;5:112 - PMID: 1234049
SPECIES: mouse, A/Sn
TUMOR: lymphoma
ECACC catalog code: 86022801
Morphology: Lymphoblast
TRANSFORMED BY: Moloney-MuLV
Description: Mouse lymphoma
Viruses: See Properties
Depositor: Prof H Harris/Dr C Madin, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: NK assays
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Induced by inoculation of Moloney leukaemia virus into a new-born A/Sn mouse. Sensitive to the cytotoxic activity of naturally occurring killer cells in mice (NK cells). Often used as target cells in NK assays.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: TIB 160, DSMZ: ACC 96
Bibliographic references:
- Eur J Immunol 1975;5:112 - PMID: 1234049
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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