Y3.Ag.1.2.3's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Y3.Ag.1.2.3 (rat, LOU, myeloma)

SPECIES: rat, LOU
TUMOR: myeloma

ECACC catalog code: 85110502
Morphology: Suspension
Description: Rat LOU myeloma
Viruses: See Properties
Depositor: Dr J Jarvis, MRC Laboratory of Molecular Biology, Cambridge, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin G (IgG) kappa light chains Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Sub-cloned from an azaguanine-resistant mutant of the Lou rat myeloma, S210. The cells secrete IgG kappa light chains.
Research council deposit: Yes; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1631

Bibliographic references:

Int J Cancer 1972;10:568 - PMID: 4668496
Nature 1973;244:42 - PMID: 4582485
Nature 1979;277:131-133 - PMID: 310519

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105