XC's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

XC (rat, Wistar, sarcoma)

SPECIES: rat, Wistar
TUMOR: sarcoma

ECACC catalog code: 88120601
Morphology: Epithelial
Endogenous viruses: contains Rous sarcoma virus
Susceptibility to viruses: Moloney-MuLV * vesic. stomatitis (Indiana)
Description: Rat Wistar sarcoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Plaque assay and virus studies: Rous sarcoma virus, MuLV

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 42
Original tumour induced in outbred Wistar rats by intramuscular injection of Rous sarcoma virus. The cells have virus genome but do not normally release infectious virus. The XC plaque assay is an indirect method of detection for MuLV in cultured cells.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 165, IZSBS: BS TCL66, DSMZ: ACC 118, ICLC: ATL96013

Bibliographic references:

Folia Biol (Praha) 1962;8:221
Nature 1960;186:980-981
Proc Natl Acad Sci U S A 1969;63:753-758 - PMID: 4186808
Virology 1970;42:1136-1139 - PMID: 4099080

XC (rat, Wistar, sarcoma)

ICLC catalog code: ATL96013
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: rat, Wistar Karyology: hypodiploid
TUMOR: sarcoma
TRANSFORMED BY: 41
Susceptibility to viruses: Moloney-MuLV * vesic. stomatitis (Indiana)
DEPOSITOR: Dr O.E. Varnier, Molecular Virology Dept, ABC, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: virology; plaque assays; used as indicator cells for detection of murine leukemia viruses

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (HBSS) + 10% FBS + 2 mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-5x104 cells/cm2. 37C, 5% CO2. Passage: 42 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as rat with AST, MD)

Availability in cell line catalogues: IZSBS: BS TCL66, ECACC: 88120601, DSMZ: ACC 118

Bibliographic references:

Chromosoma 1970;31:51-60 - PMID: 4321325
Folia Biol (Praha) 1962;8:221
Nature 1960;186:980-981
Proc Natl Acad Sci U S A 1969;63:753-758 - PMID: 4186808
Virology 1970;42:1136-1139 - PMID: 4099080

XC (rat, Wistar, sarcoma)

IZSBS catalog code: BS TCL66
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: rat, Wistar Karyology: hypodiploid
TUMOR: sarcoma
Endogenous viruses: contains Rous sarcoma virus
Susceptibility to viruses: Moloney-MuLV * vesic. stomatitis (Indiana)
PROPERTIES: virology; plaque assays

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (HBSS) + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Derived from tumor induced by intramuscolar injection of the Prague strain of Rous sarcoma virus

Availability in cell line catalogues: ATCC: CCL 165, ECACC: 88120601, DSMZ: ACC 118, ICLC: ATL96013

XC (rat, Wistar, sarcoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: rat, Wistar Karyology: hypodiploid
TUMOR: sarcoma
Endogenous viruses: contains Rous sarcoma virus
Susceptibility to viruses: Moloney-MuLV * vesic. stomatitis (Indiana)
PROPERTIES: virology; plaque assays

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)

Used for revealing of murine leukemia viruses At the moment the availability of this cell line is not sure

Availability in cell line catalogues: ATCC: CCL 165, IZSBS: BS TCL66, ECACC: 88120601, DSMZ: ACC 118, ICLC: ATL96013

Bibliographic references:

Folia Biol (Praha) 1962;8:221
Nature 1960;186:980-981
Proc Natl Acad Sci U S A 1969;63:753-758 - PMID: 4186808
Virology 1970;42:1136-1139 - PMID: 4099080

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105