SPECIES: rat, Wistar
TUMOR: sarcoma
DSMZ catalog code: ACC 118
Morphology: epithelial-like, small cells growing as monolayers
Endogenous viruses: contains Rous sarcoma virus
Susceptibility to viruses: Moloney-MuLV * vesic. stomatitis (Indiana)
Description: rat sarcoma the original tumor was induced in a Wistar rat by intramuscular injection of Rous sarcoma virus; cells have been used as indicator cells for detection of murine leukemia viruses confirmed as rat with IEF of AST, NP
Viruses: ELISA. reverse transcriptase positive
Depositor: obtained from ATCC (CCL 165), Rockville, Maryland, USA
General Restrictions
Available in the following LABORATORY:
-
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS + non-essential amino acids split confluent culture 1:4 every 3 days using trysin/EDTA at 37C with 5% CO2 about 1-2 x 107 cells/80 cm 2;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as rat with IEF of AST, NP)
Availability in cell line catalogues: ATCC: CCL 165, IZSBS: BS TCL66, ECACC: 88120601, ICLC: ATL96013
Bibliographic references:
- Folia Biol (Praha) 1962;8:221
- Nature 1960;186:980-981
- Proc Natl Acad Sci U S A 1969;63:753-758 - PMID: 4186808
- Virology 1970;42:1136-1139 - PMID: 4099080
SPECIES: rat, Wistar
TUMOR: sarcoma
ECACC catalog code: 88120601
Morphology: Epithelial
Endogenous viruses: contains Rous sarcoma virus
Susceptibility to viruses: Moloney-MuLV * vesic. stomatitis (Indiana)
Description: Rat Wistar sarcoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Plaque assay and virus studies: Rous sarcoma virus, MuLV
Available in the following LABORATORY:
-
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 42
Original tumour induced in outbred Wistar rats by intramuscular injection of Rous sarcoma virus. The cells have virus genome but do not normally release infectious virus. The XC plaque assay is an indirect method of detection for MuLV in cultured cells.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 165, IZSBS: BS TCL66, DSMZ: ACC 118, ICLC: ATL96013
Bibliographic references:
- Folia Biol (Praha) 1962;8:221
- Nature 1960;186:980-981
- Proc Natl Acad Sci U S A 1969;63:753-758 - PMID: 4186808
- Virology 1970;42:1136-1139 - PMID: 4099080
ICLC catalog code: ATL96013
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: rat, Wistar Karyology: hypodiploid
TUMOR: sarcoma
TRANSFORMED BY: 41
Susceptibility to viruses: Moloney-MuLV * vesic. stomatitis (Indiana)
DEPOSITOR: Dr O.E. Varnier, Molecular Virology Dept, ABC, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: virology; plaque assays; used as indicator cells for detection of murine leukemia viruses
Available in the following LABORATORY:
-
IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (HBSS) + 10% FBS + 2 mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-5x104 cells/cm2. 37C, 5% CO2. Passage: 42 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as rat with AST, MD)
Availability in cell line catalogues: IZSBS: BS TCL66, ECACC: 88120601, DSMZ: ACC 118
Bibliographic references:
- Chromosoma 1970;31:51-60 - PMID: 4321325
- Folia Biol (Praha) 1962;8:221
- Nature 1960;186:980-981
- Proc Natl Acad Sci U S A 1969;63:753-758 - PMID: 4186808
- Virology 1970;42:1136-1139 - PMID: 4099080
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: rat, Wistar Karyology: hypodiploid
TUMOR: sarcoma
Endogenous viruses: contains Rous sarcoma virus
Susceptibility to viruses: Moloney-MuLV * vesic. stomatitis (Indiana)
PROPERTIES: virology; plaque assays
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 165, IZSBS: BS TCL66, ECACC: 88120601, DSMZ: ACC 118, ICLC: ATL96013
Bibliographic references:
- Folia Biol (Praha) 1962;8:221
- Nature 1960;186:980-981
- Proc Natl Acad Sci U S A 1969;63:753-758 - PMID: 4186808
- Virology 1970;42:1136-1139 - PMID: 4099080
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
|
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
|
