Vero's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Vero (monkey, African green, kidney)

SPECIES: monkey, African green
TISSUE/ORGAN: kidney

ECACC catalog code: 84113001
Morphology: Fibroblast-like
Susceptibility to viruses: adenovirus 12 * African swine fever virus * arbovirus * bluetongue virus * bovine leucosis * echovirus * Getah arbovirus * herpes simplex * influenza * orbivirus * orthomixovirus * paramyxovirus * poliovirus 3 * porcine epidemic diarrhea virus * reovirus * rubella
Description: Monkey African Green kidney
Viruses: See Properties
Depositor: Dr B Thornton, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: polio, rubella, arboviruses and reoviruses

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 12; Karyotype: 2n = 60, modal No 58
Established from the kidney of a normal adult African Green monkey. Susceptible to a wide range of viruses including polio, rubella, arboviruses and reoviruses.
Research council deposit: No; Price Code: A;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 81, IZSBS: BS CL 86, ICLC: ATL95005

Bibliographic references:

Acta Pathol Microbiol Scand Sec B 1981;89:179
Am J Vet Res 1980;41:1357 - PMID: 7004279
Arch GVS Virusforsch 1969;27:379 - PMID: 4313210
Avian Dis 1988;32:298 - PMID: 2840882
Intervirology 1983;20:181 - PMID: 6654644
J Clin Microbiol 1988;26:234
Microbiol Immunol 1979;23:229
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

Vero (monkey, African green, kidney)

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 12; Karyotype: 2n = 60, modal No 58
Established from the kidney of a normal adult African Green monkey. Susceptible to a wide range of viruses including polio, rubella, arboviruses and reoviruses.
Research council deposit: No; Price Code: A;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 81, IZSBS: BS CL 86, ICLC: ATL95005

Bibliographic references:

Acta Pathol Microbiol Scand Sec B 1981;89:179
Am J Vet Res 1980;41:1357 - PMID: 7004279
Arch GVS Virusforsch 1969;27:379 - PMID: 4313210
Avian Dis 1988;32:298 - PMID: 2840882
Intervirology 1983;20:181 - PMID: 6654644
J Clin Microbiol 1988;26:234
Microbiol Immunol 1979;23:229
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

Vero (monkey, African green, kidney)

IZSBS catalog code: BS CL 86
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: monkey, African green Karyology: 2n = 60 in 50 cells
TISSUE/ORGAN: kidney
Susceptibility to viruses: adenovirus 12 * African swine fever virus * arbovirus * bluetongue virus * bovine leucosis * infectious bursal disease virus * orthomixovirus * paramyxovirus * poliovirus 3 * porcine epidemic diarrhea virus * rubella
DEPOSITOR: obtained from ATCC
PROPERTIES: virology

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 151 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as monkey with NP, G6PD, MD, MPI, AST)

Availability in cell line catalogues: ATCC: CCL 81, ECACC: 84113001, ICLC: ATL95005, ECACC: 88020401

Bibliographic references:

Acta Pathol Microbiol Scand Sec B 1981;89:179
Am J Vet Res 1980;41:1357 - PMID: 7004279
Avian Dis 1988;32:298 - PMID: 2840882
Intervirology 1983;20:181 - PMID: 6654644
Microbiol Immunol 1979;23:299
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

Vero (monkey, African green, kidney)

Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: monkey, African green Karyology: modal number 58
TISSUE/ORGAN: kidney
Susceptibility to viruses: adenovirus 12 * African swine fever virus * arbovirus * bluetongue virus * bovine leucosis * echovirus * Getah arbovirus * herpes simplex * influenza * orbivirus * orthomixovirus * paramyxovirus * poliovirus 3 * porcine epidemic diarrhea virus * reovirus * rubella
PROPERTIES: virology; virus titration; virus replication; plaque assays; bacterial cytotoxicity

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)
mycoplasma negative
Epithelial-like morphology. At the moment the availability of this cell line is not sure

Availability in cell line catalogues: ATCC: CCL 81, IZSBS: BS CL 86, ECACC: 84113001, ICLC: ATL95005, ECACC: 88020401

Bibliographic references:

Acta Pathol Microbiol Scand Sec B 1981;89:179
Am J Vet Res 1980;41:1357 - PMID: 7004279
Arch GVS Virusforsch 1969;27:379 - PMID: 4313210
Avian Dis 1988;32:298 - PMID: 2840882
Intervirology 1983;20:181 - PMID: 6654644
J Clin Microbiol 1988;26:234
Microbiol Immunol 1979;23:229
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

Vero (monkey, African green, kidney)

Available in the following LABORATORY:

Universita' degli Studi (PRUMB, Parma)
Medium 199 + 5% FBS + 2mM L-Glutamine; Freezing medium: Medium 199 + 10% FBS + 10% DMSO; 37C, 5% CO2 Passage: 135

Availability in cell line catalogues: ATCC: CCL 81, IZSBS: BS CL 86, ECACC: 84113001, ICLC: ATL95005, ECACC: 88020401

Bibliographic references:

Acta Pathol Microbiol Scand Sec B 1981;89:179
Am J Vet Res 1980;41:1357 - PMID: 7004279
Arch GVS Virusforsch 1969;27:379 - PMID: 4313210
Avian Dis 1988;32:298 - PMID: 2840882
Intervirology 1983;20:181 - PMID: 6654644
J Clin Microbiol 1988;26:234
Microbiol Immunol 1979;23:229
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

Vero (monkey, African green, kidney)

Monoclonal continuous culture, grown as monolayer
fibroblast morphology
SPECIES: monkey, African green Karyology: modal number 58
TISSUE/ORGAN: kidney
Susceptibility to viruses: adenovirus 12 * African swine fever virus * arbovirus * bluetongue virus * bovine leucosis * echovirus * Getah arbovirus * herpes simplex * influenza * orbivirus * orthomixovirus * paramyxovirus * poliovirus 3 * porcine epidemic diarrhea virus * reovirus * rubella
PROPERTIES: virology; virus titration; virus replication; plaque assays; bacterial cytotoxicity

Available in the following LABORATORY:

Dip. Biologia Molecolare, Universita' degli Studi (SIUVI, Siena)
MEM modified + 5% FBS + Antibiotics + NaHCO3; Freezing medium: MEM modified + 20% FBS + 7.5% DMSO; 37C, 5% CO2, split 1-4 , mycoplasma negative, enzyme immunoassay (EIA)

Availability in cell line catalogues: ATCC: CCL 81, IZSBS: BS CL 86, ECACC: 84113001, ICLC: ATL95005, ECACC: 88020401

Bibliographic references:

Acta Pathol Microbiol Scand Sec B 1981;89:179
Am J Vet Res 1980;41:1357 - PMID: 7004279
Arch GVS Virusforsch 1969;27:379 - PMID: 4313210
Avian Dis 1988;32:298 - PMID: 2840882
Intervirology 1983;20:181 - PMID: 6654644
J Clin Microbiol 1988;26:234
Microbiol Immunol 1979;23:229
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

Vero (monkey, African green, kidney)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CCL 81, IZSBS: BS CL 86, ECACC: 84113001, ICLC: ATL95005, ECACC: 88020401

Bibliographic references:

Acta Pathol Microbiol Scand Sec B 1981;89:179
Am J Vet Res 1980;41:1357 - PMID: 7004279
Arch GVS Virusforsch 1969;27:379 - PMID: 4313210
Avian Dis 1988;32:298 - PMID: 2840882
Intervirology 1983;20:181 - PMID: 6654644
J Clin Microbiol 1988;26:234
Microbiol Immunol 1979;23:229
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105