Vero's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Vero (monkey, African green, kidney)

SPECIES: monkey, African green
TISSUE/ORGAN: kidney

ECACC catalog code: 88020401
Morphology: Fibroblast-like
Description: Monkey African Green kidney
Viruses: See Properties
Depositor: WHO, Geneva, Switzerland
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Vaccine Production

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). A full description of the recommended subculture methods and documentation of the cell bank production is provided with the cells.
Passages: 137; Karyotype: 2n=60
WHO working cell bank of 1,000 ampoules deposited at ECACC. Derived from an original American Type Culture Collection (ATCC) ampoule. Available free of charge to organisations producing vaccines.
Research council deposit: No; Price Code: B;

Availability in cell line catalogues: IZSBS: BS CL 86, ICLC: ATL95005

Vero (monkey, African green, kidney)

ICLC catalog code: ATL95005
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: monkey, African green (adult) Karyology: modal number 58
TISSUE/ORGAN: kidney
Susceptibility to viruses: adenovirus 12 * African swine fever virus * arbovirus * bluetongue virus * bovine leucosis * echovirus * Getah arbovirus * herpes simplex * influenza * orbivirus * orthomixovirus * paramyxovirus * poliovirus 3 * porcine epidemic diarrhea virus * reovirus * rubella
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: indicator line for mycoplasma testing; virology; virus titration; virus replication; plaque assays; bacterial cytotoxicity.

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:20 using trypsin/EDTA; seed at 1-3x104 cells/cm2. 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as monkey with AST, MD, NP, PepB)

Availability in cell line catalogues: IZSBS: BS CL 86, ECACC: 84113001, ECACC: 88020401

Bibliographic references:

Kitasato Arc Exp Med 1964;37:27-42 - PMID: 5833688
Nature 1967;216:271
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

Vero (monkey, African green, kidney)

IZSBS catalog code: BS CL 86
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: monkey, African green Karyology: 2n = 60 in 50 cells
TISSUE/ORGAN: kidney
Susceptibility to viruses: adenovirus 12 * African swine fever virus * arbovirus * bluetongue virus * bovine leucosis * infectious bursal disease virus * orthomixovirus * paramyxovirus * poliovirus 3 * porcine epidemic diarrhea virus * rubella
DEPOSITOR: obtained from ATCC
PROPERTIES: virology

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 151 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as monkey with NP, G6PD, MD, MPI, AST)

Availability in cell line catalogues: ATCC: CCL 81, ECACC: 84113001, ICLC: ATL95005, ECACC: 88020401

Bibliographic references:

Acta Pathol Microbiol Scand Sec B 1981;89:179
Am J Vet Res 1980;41:1357 - PMID: 7004279
Avian Dis 1988;32:298 - PMID: 2840882
Intervirology 1983;20:181 - PMID: 6654644
Microbiol Immunol 1979;23:299
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

Vero (monkey, African green, kidney)

Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: monkey, African green Karyology: modal number 58
TISSUE/ORGAN: kidney
Susceptibility to viruses: adenovirus 12 * African swine fever virus * arbovirus * bluetongue virus * bovine leucosis * echovirus * Getah arbovirus * herpes simplex * influenza * orbivirus * orthomixovirus * paramyxovirus * poliovirus 3 * porcine epidemic diarrhea virus * reovirus * rubella
PROPERTIES: virology; virus titration; virus replication; plaque assays; bacterial cytotoxicity

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)
mycoplasma negative
Epithelial-like morphology. At the moment the availability of this cell line is not sure

Availability in cell line catalogues: ATCC: CCL 81, IZSBS: BS CL 86, ECACC: 84113001, ICLC: ATL95005, ECACC: 88020401

Bibliographic references:

Acta Pathol Microbiol Scand Sec B 1981;89:179
Am J Vet Res 1980;41:1357 - PMID: 7004279
Arch GVS Virusforsch 1969;27:379 - PMID: 4313210
Avian Dis 1988;32:298 - PMID: 2840882
Intervirology 1983;20:181 - PMID: 6654644
J Clin Microbiol 1988;26:234
Microbiol Immunol 1979;23:229
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

Vero (monkey, African green, kidney)

Available in the following LABORATORY:

Universita' degli Studi (PRUMB, Parma)
Medium 199 + 5% FBS + 2mM L-Glutamine; Freezing medium: Medium 199 + 10% FBS + 10% DMSO; 37C, 5% CO2 Passage: 135

Availability in cell line catalogues: ATCC: CCL 81, IZSBS: BS CL 86, ECACC: 84113001, ICLC: ATL95005, ECACC: 88020401

Bibliographic references:

Acta Pathol Microbiol Scand Sec B 1981;89:179
Am J Vet Res 1980;41:1357 - PMID: 7004279
Arch GVS Virusforsch 1969;27:379 - PMID: 4313210
Avian Dis 1988;32:298 - PMID: 2840882
Intervirology 1983;20:181 - PMID: 6654644
J Clin Microbiol 1988;26:234
Microbiol Immunol 1979;23:229
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

Vero (monkey, African green, kidney)

Monoclonal continuous culture, grown as monolayer
fibroblast morphology
SPECIES: monkey, African green Karyology: modal number 58
TISSUE/ORGAN: kidney
Susceptibility to viruses: adenovirus 12 * African swine fever virus * arbovirus * bluetongue virus * bovine leucosis * echovirus * Getah arbovirus * herpes simplex * influenza * orbivirus * orthomixovirus * paramyxovirus * poliovirus 3 * porcine epidemic diarrhea virus * reovirus * rubella
PROPERTIES: virology; virus titration; virus replication; plaque assays; bacterial cytotoxicity

Available in the following LABORATORY:

Dip. Biologia Molecolare, Universita' degli Studi (SIUVI, Siena)
MEM modified + 5% FBS + Antibiotics + NaHCO3; Freezing medium: MEM modified + 20% FBS + 7.5% DMSO; 37C, 5% CO2, split 1-4 , mycoplasma negative, enzyme immunoassay (EIA)

Availability in cell line catalogues: ATCC: CCL 81, IZSBS: BS CL 86, ECACC: 84113001, ICLC: ATL95005, ECACC: 88020401

Bibliographic references:

Acta Pathol Microbiol Scand Sec B 1981;89:179
Am J Vet Res 1980;41:1357 - PMID: 7004279
Arch GVS Virusforsch 1969;27:379 - PMID: 4313210
Avian Dis 1988;32:298 - PMID: 2840882
Intervirology 1983;20:181 - PMID: 6654644
J Clin Microbiol 1988;26:234
Microbiol Immunol 1979;23:229
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

Vero (monkey, African green, kidney)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CCL 81, IZSBS: BS CL 86, ECACC: 84113001, ICLC: ATL95005, ECACC: 88020401

Bibliographic references:

Acta Pathol Microbiol Scand Sec B 1981;89:179
Am J Vet Res 1980;41:1357 - PMID: 7004279
Arch GVS Virusforsch 1969;27:379 - PMID: 4313210
Avian Dis 1988;32:298 - PMID: 2840882
Intervirology 1983;20:181 - PMID: 6654644
J Clin Microbiol 1988;26:234
Microbiol Immunol 1979;23:229
Nippon Rinsho 1963;21:1209
Proc Soc Exp Biol Med 1967;125:602 - PMID: 4961492

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105