T47D's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

T47D (human, breast, carcinoma, ductal)

ICLC catalog code: HTL98003
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (female, 54 years old)
TISSUE/ORGAN: breast
TUMOR: carcinoma, ductal
DEPOSITOR: Dr U. Pfeffer, Molecular Biology Dept IST at ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: receptors for a variety of steroids and calcitonin

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma contamination eliminated with BM-cyclin; negative, HOECHST and PCR
Validated by: identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X; vWA: 14,15; FGA: 23; TH01: 6; D18S51: 17; D21S11: 28,31; D8S1179: 13) * isoenzymes (confirmed as human with MD, NP)

Availability in cell line catalogues: ECACC: 85102201

Bibliographic references:

Biochem Biophys Res Commun 1981;101:1131-1138 - PMID: 6272774
Biochem J 1981;200:315-320 - PMID: 6896147

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105