SW620's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

SW620 (human, Caucasian, colon, adenocarcinoma)

ICLC catalog code: HTL99015
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 51 years old)
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
highly tumorigenic in nude mice
DEPOSITOR: Dr U. Pfeffer, Molecular Biology Unit, IST at ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: Products: carcinoembryonic antigen (CEA), transforming growth factor alpha, keratin, matrilysin; p53 (G>A mutation in codon 273)

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent culture 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)

Bibliographic references:

Cancer Res 1976;36:4562-4569 - PMID: 1000501
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105