SPECIES: mouse / mouse
TUMOR: myeloma
DSMZ catalog code: ACC 146
Morphology: single, round cells in suspension
Transformed by: Mutations Back To Mutations WebPage
Description: mouse myeloma SP2/0-AG14 is a hybrid between BALB/c spleen cells and the myeloma cell line P3X63AG8; cells were described as not synthesizing or secreting immunoglobulin chains, being resistant to azaguanine at 20 ęg/ml, and not growing in HAT medium; cell line is used as a fusion partner for the production of hybridomas secreting specific monoclonal antibodies confirmed as mouse with IEF of LDH, NP, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. G. Koehler, Max-Planck-Institute, Freiburg, Germany
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
92.5% Iscove's MDM + 7.5% FBS + 2 mM L-glutamine maintain at 0.1-0.5 x 106 cells/ml; split 1:3 to 1:10 every 2-4 days; seed out at ca. 0.5 x 106 cells/ml at 37C with 7.5% CO2 maximal density at 0.5-1.0 x 106 cells/ml;
doubling time of ca. 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as mouse with IEF of LDH, NP, PEP B)
Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, ECACC: 85072401
Bibliographic references:
- J Immunol 1981;126:317-321 - PMID: 6935293
- Nature 1978;276:269-270 - PMID: 714156
SPECIES: mouse, BALB/c
TISSUE/ORGAN: hybrid
TUMOR: myeloma
ECACC catalog code: 85072401
Morphology: Suspension
Transformed by: Mutations Back To Mutations WebPage
Description: Mouse x Mouse hybridoma, non-producing
Viruses: See Properties
Depositor: Dr P Wilton-Smith, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Fusion partner
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 or DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C. HAT sensitive.
Sp2/0-Ag14 is a non-Ig-secreting or synthesizing line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Resistant to 8-azaguanine at 20ug/ml and does not survive in HAT containing media.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, DSMZ: ACC 146
Bibliographic references:
- J Immunol 1981;126:317-321 - PMID: 6935293
- Nature 1978;276:269-270 - PMID: 714156
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: mouse, BALB/c (female)
TISSUE/ORGAN: hybrid
TUMOR: myeloma
Original line: P3X63Ag8 x BALB/c mouse spleen cell
Transformed by: fusion with anti SRBC spleen cells by PEG
tumorigenic in syngeneic animals
PROPERTIES: Ig non synthesized; resistant to 8-azaguanine; fusion partner; monoclonal antibody prod.
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, ECACC: 85072401, DSMZ: ACC 146
Bibliographic references:
- J Immunol 1981;126:317-321 - PMID: 6935293
- Nature 1978;276:269-270 - PMID: 714156
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: mouse, BALB/c (female)
TISSUE/ORGAN: hybrid
TUMOR: myeloma
Original line: P3X63Ag8 x BALB/c mouse spleen cell
Transformed by: fusion with anti SRBC spleen cells by PEG
tumorigenic in syngeneic animals
PROPERTIES: Ig non synthesized; resistant to 8-azaguanine; fusion partner; monoclonal antibody prod.
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, ECACC: 85072401, DSMZ: ACC 146
Bibliographic references:
- J Immunol 1981;126:317-321 - PMID: 6935293
- Nature 1978;276:269-270 - PMID: 714156
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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