SK-N-SH's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

SK-N-SH (human, Caucasian, bone marrow, neuroblastoma)

Monoclonal continuous culture, grown as monolayer
neuroblast-like or epithelial-like morphology
SPECIES: human, Caucasian (female, 4 years old) Karyology: modal number 47
TISSUE/ORGAN: bone marrow
TUMOR: neuroblastoma
tumorigenic in nude mice
PROPERTIES: biochemical markers; receptor; chemotherapeutic agents; nucleic acids; neural tissue; cytotoxicity

Available in the following LABORATORY:

Clinica Pediatrica, Ospedale S.Orsola-Malpighi (BOHIC, Bologna)
RPMI 1640 + 10% FBS + 1% non-essential amino acids; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-12.5 , mycoplasma negative, DAPI and HOECHST
Isolated from metastasis to bone marrow
Validated by: isoenzymes

Availability in cell line catalogues: ATCC: HTB 11, ECACC: 86012802, ICLC: HTL96019

Bibliographic references:

Cancer Res 1973;33:2643-2652 - PMID: 4748425
In Vitro 1973;7:410
Proc Natl Acad Sci U S A 1978;75:3965-3969 - PMID: 279013

SK-N-SH (human, Caucasian, bone marrow, neuroblastoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: bone marrow
TUMOR: neuroblastoma

ECACC catalog code: 86012802
Morphology: Epithelial
Description: Human Caucasian neuroblastoma
Viruses: See Properties
Depositor: Dr M Shaw, ICI Pharmaceuticals Ltd, UK
No restrictions. Patent: None Specified By Depositor

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12 or DMEM or EMEM (EBSS) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 35; Karyotype: 2n = 46, modal No 47
Established from a bone marrow metastasis from a 4-year-old caucasian female suffering with neuroblastoma. The cells have been used as a target cell in cell-mediated cytoxicity tests.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: HTB 11, ICLC: HTL96019

Bibliographic references:

Cancer Res 1973;33:2643-2652 - PMID: 4748425
In Vitro 1973;7:410
Proc Natl Acad Sci U S A 1978;75:3965-3969 - PMID: 279013

SK-N-SH (human, Caucasian, neuroblastoma)

ICLC catalog code: HTL96019
Continuous culture, grown as monolayer
neuroblast-like and epithelial-like morphology
SPECIES: human, Caucasian (female, 4 years old) Karyology: modal number 47
TUMOR: neuroblastoma
tumorigenic in nude mice
DEPOSITOR: Dr P. Montaldo, G. Gaslini Institute, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: receptor cell line in cell-mediated cytotoxicity

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, G6PD)

Availability in cell line catalogues: ECACC: 86012802

Bibliographic references:

Cancer Res 1973;33:2643-2652 - PMID: 4748425
In Vitro 1973;7:410
Proc Natl Acad Sci USA 1978;75:3965-3969 - PMID: 279013

SK-N-SH (human, Caucasian, bone marrow, neuroblastoma)

Continuous culture, grown as monolayer
neuroblast-like or epithelial-like morphology
SPECIES: human, Caucasian (female, 4 years old) Karyology: modal number 47
TISSUE/ORGAN: bone marrow
TUMOR: neuroblastoma
tumorigenic in nude mice
PROPERTIES: biochemical markers; receptor; chemotherapeutic agents; nucleic acids; neural tissue; cytotoxicity

Available in the following LABORATORY:

Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISA, Milano)
MEM + 10% FBS + 1% non-essential amino acids; Freezing medium: MEM + 30% FBS + NEAA + NaP + 10% DMSO; 37C, 5% CO2

Availability in cell line catalogues: ATCC: HTB 11, ECACC: 86012802, ICLC: HTL96019

Bibliographic references:

Cancer Res 1973;33:2643-2652 - PMID: 4748425
In Vitro 1973;7:410
Proc Natl Acad Sci U S A 1978;75:3965-3969 - PMID: 279013

SK-N-SH (human, Caucasian, bone marrow, neuroblastoma)

Available in the following LABORATORY:

Istituto Endocrinologia, Universita' degli Studi (MIUEP, Milano)
MEM + 10% FBS; Freezing medium: MEM + 5% DMSO; 37C, 5% CO2, split 1-12 Passage: 41 , mycoplasma negative

Availability in cell line catalogues: ATCC: HTB 11, ECACC: 86012802, ICLC: HTL96019

Bibliographic references:

Cancer Res 1973;33:2643-2652 - PMID: 4748425
In Vitro 1973;7:410
Proc Natl Acad Sci U S A 1978;75:3965-3969 - PMID: 279013

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105