SK-N-MC's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

SK-N-MC (human, Caucasian, neuroblastoma)

Monoclonal continuous culture, grown as monolayer
neuroblast-like morphology
SPECIES: human, Caucasian (female, 14 years old)
TUMOR: neuroblastoma
tumorigenic in nude mice
PROPERTIES: biochemical markers; catecholamine prod.; receptor; chemotherapeutic agents; neural tissue

Available in the following LABORATORY:

Clinica Pediatrica, Ospedale S.Orsola-Malpighi (BOHIC, Bologna)
RPMI 1640 + 10% FBS + 1% non-essential amino acids; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-6 , mycoplasma negative, DAPI and HOECHST
Isolated from metastasis to supra-orbital area
Validated by: isoenzymes

Availability in cell line catalogues: ATCC: HTB 10, ECACC: 90022302, DSMZ: ACC 203

Bibliographic references:

Cancer Genet Cytogenet 1994;76:19-22 - PMID: 8076344
Cancer Res 1973;33:2643-2652 - PMID: 4748425
In Vitro 1973;8:410

SK-N-MC (human, Caucasian, neuroblastoma)

Continuous culture, grown as monolayer
neuroblast-like morphology
SPECIES: human, Caucasian (female, 14 years old)
TUMOR: neuroblastoma
tumorigenic in nude mice
PROPERTIES: biochemical markers; catecholamine prod.; receptor; chemotherapeutic agents; neural tissue

Available in the following LABORATORY:

Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISA, Milano)
MEM + 10% FBS + 1% non-essential amino acids; Freezing medium: Culture medium + 30% FBS + 10% DMSO; 37C, 5% CO2

Availability in cell line catalogues: ATCC: HTB 10, ECACC: 90022302, DSMZ: ACC 203

Bibliographic references:

Cancer Genet Cytogenet 1994;76:19-22 - PMID: 8076344
Cancer Res 1973;33:2643-2652 - PMID: 4748425
In Vitro 1973;8:410

SK-N-MC (human, Caucasian, neuroblastoma)

Available in the following LABORATORY:

Istituto Endocrinologia, Universita' degli Studi (MIUEP, Milano)
MEM + 10% FBS; Freezing medium: MEM + 5% DMSO; 37C, 5% CO2, split 1-25 Passage: 48 , mycoplasma negative
Epithelial-like morphology

Availability in cell line catalogues: ATCC: HTB 10, ECACC: 90022302, DSMZ: ACC 203

Bibliographic references:

Cancer Genet Cytogenet 1994;76:19-22 - PMID: 8076344
Cancer Res 1973;33:2643-2652 - PMID: 4748425
In Vitro 1973;8:410

SK-N-MC (human, Caucasian, neuroblastoma)

Available in the following LABORATORY:

Universita' La Sapienza (RMUPG, Roma)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative

Availability in cell line catalogues: ATCC: HTB 10, ECACC: 90022302, DSMZ: ACC 203

Bibliographic references:

Cancer Genet Cytogenet 1994;76:19-22 - PMID: 8076344
Cancer Res 1973;33:2643-2652 - PMID: 4748425
In Vitro 1973;8:410

SK-N-MC (human, Caucasian, neuroblastoma)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: HTB 10, ECACC: 90022302, DSMZ: ACC 203

Bibliographic references:

Cancer Genet Cytogenet 1994;76:19-22 - PMID: 8076344
Cancer Res 1973;33:2643-2652 - PMID: 4748425
In Vitro 1973;8:410

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105