SPECIES: monkey, African green
TISSUE/ORGAN: kidney
ECACC catalog code: 90092601
Morphology: Fibroblast
Susceptibility to viruses: Coxsackie * echovirus * many types of viruses * poliovirus * reovirus * rotavirus
Description: Monkey African green kidney
Viruses: See Properties
Depositor: Dr S Clark, CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: polio, Echo, Coxsackie and Reo viruses
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:8 to 1:20 i.e. seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin EDTA; 5% CO2; 37C.
Passages: 80;
Used for the isolation of water borne viruses e.g. polio 1, 2, 3, Echo 3, 6, 7, 9, 12, 27, Coxsackie A9 + B1, 2, 3 and Reo 1.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: IZSBS: BS CL 7
Bibliographic references:
- Append Environ Microbiol 1986;51:790
- Arch Gesamte Virusforsch 1970;32:389 - PMID: 4993582
- Health Lab Sci 1974;110:275 - PMID: 4369820
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
Susceptibility to viruses: Coxsackie * echovirus * many types of viruses * poliovirus * reovirus * rotavirus
PROPERTIES: chlamidia growth substrate; virology
Available in the following LABORATORY:
Availability in cell line catalogues: IZSBS: BS CL 7, ECACC: 90092601
Bibliographic references:
- Append Environ Microbiol 1986;51:790
- Arch Gesamte Virusforsch 1970;32:389 - PMID: 4993582
- Health Lab Sci 1974;110:275 - PMID: 4369820
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
Susceptibility to viruses: Coxsackie * echovirus * many types of viruses * poliovirus * reovirus * rotavirus
PROPERTIES: chlamidia growth substrate; virology
Available in the following LABORATORY:
-
Universita' degli Studi (PRUMB, Parma)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: MEM + 20% FBS + 10% DMSO; 37C, 5% CO2 Passage: 85
Susceptibility to viruses: Echovirus type 3,6,7,9,11,12,27; Coxsackievirus A9,B1,B2,B3; Poliovirus 1,2,3; Reovirus 1; Rotavirus SA11
Availability in cell line catalogues: IZSBS: BS CL 7, ECACC: 90092601
Bibliographic references:
- Append Environ Microbiol 1986;51:790
- Arch Gesamte Virusforsch 1970;32:389 - PMID: 4993582
- Health Lab Sci 1974;110:275 - PMID: 4369820
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
Susceptibility to viruses: Coxsackie * echovirus * many types of viruses * poliovirus * reovirus * rotavirus
PROPERTIES: chlamidia growth substrate; virology
Available in the following LABORATORY:
Availability in cell line catalogues: IZSBS: BS CL 7, ECACC: 90092601
Bibliographic references:
- Append Environ Microbiol 1986;51:790
- Arch Gesamte Virusforsch 1970;32:389 - PMID: 4993582
- Health Lab Sci 1974;110:275 - PMID: 4369820
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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