Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 52 years old) Karyology: hyperdiploid to hypotriploid
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: bFGF prod.; tumorigenicity
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 52, ECACC: 91091816, DSMZ: ACC 141, ICLC: HTL10001
Bibliographic references:
- Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
- Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047
SPECIES: human, Caucasian
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma
ECACC catalog code: 91091816
Morphology: Endothelial
Description: Human liver adenocarcinoma
Viruses: See Properties
Depositor: Not registered
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
-
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 35;
Karyotype: Hyperdiploid to hypotriploid
Derived from an ascites sample from a 52 year old male suffering from adenocarcinoma of the liver. The cells have now been shown to be endothelial in origin (In Vitro 1992;28A:136).
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: HTB 52, DSMZ: ACC 141, ICLC: HTL10001
Bibliographic references:
- Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
- Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047
ICLC catalog code: HTL10001
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male)
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma
DEPOSITOR: A M Bassi e S Penco, DIMES, General Pathology, Cell Cultures Lab, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 1% non-essential amino acids; Freezing medium: Culture medium 45% + 50% FCS + 5% DMSO; split confluent cultures 1:5 - 1:10 using trypsin/EDTA, 37C, 5% CO2 , mycoplasma contamination eliminated with plasmocin; negative, HOECHST and PCR
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: ECACC: 91091816, DSMZ: ACC 141
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 52 years old) Karyology: hyperdiploid to hypotriploid
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: bFGF prod.; tumorigenicity
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 52, ECACC: 91091816, DSMZ: ACC 141, ICLC: HTL10001
Bibliographic references:
- Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
- Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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