Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 52 years old) Karyology: hyperdiploid to hypotriploid
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: bFGF prod.; tumorigenicity
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 52, ECACC: 91091816, DSMZ: ACC 141, ICLC: HTL10001
Bibliographic references:
- Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
- Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047
SPECIES: human, Caucasian
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma
DSMZ catalog code: ACC 141
Morphology: epithelial-like cells growing as monolayer
Description: human liver adenocarcinoma established from the ascites of a 52-year-old Caucasian man in 1971 confirmed as human with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. Kirchner, Medical School of Hannover, Hannover, Germany
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS split confluent culture 1:10 to 1:20 about 1-2 times a week using trypsin/EDTA; cells grow fast; seed out at ca. 3-4 x 106 cells/80 cm 2 at 37C with 5% CO2 about 15-18 x 106 cells/175 cm 2;
doubling time of ca. 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotriploid karyotype with 9% polyploidy; 62/63(54-64)<3n>X/XX, -Y, -3, -4, +6, +7, -9, -13, -13, -13, -14, -15, -16, -16, +17, -18, +19, +20, -21, +22, +mar, der(1)t(1;?)(q21;?), t(2;20)(p11;p11), del(6)(q25), der(14)t(14;?) * cytogenetics 2 (i(14q), der(19)t(19;?)(p13;?); sidelines carry t(14q15q) for i(14q) and der(12)t(12;?)(p12.3;?)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin++, desmin-, endothel-, GFAP-, neurofilament-, vimentin++) * isoenzymes (confirmed as human with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HTB 52, ECACC: 91091816, ICLC: HTL10001
Bibliographic references:
- Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
- Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047
SPECIES: human, Caucasian
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma
ECACC catalog code: 91091816
Morphology: Endothelial
Description: Human liver adenocarcinoma
Viruses: See Properties
Depositor: Not registered
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 35;
Karyotype: Hyperdiploid to hypotriploid
Derived from an ascites sample from a 52 year old male suffering from adenocarcinoma of the liver. The cells have now been shown to be endothelial in origin (In Vitro 1992;28A:136).
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: HTB 52, DSMZ: ACC 141, ICLC: HTL10001
Bibliographic references:
- Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
- Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047
ICLC catalog code: HTL10001
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male)
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma
DEPOSITOR: A M Bassi e S Penco, DIMES, General Pathology, Cell Cultures Lab, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 1% non-essential amino acids; Freezing medium: Culture medium 45% + 50% FCS + 5% DMSO; split confluent cultures 1:5 - 1:10 using trypsin/EDTA, 37C, 5% CO2 , mycoplasma contamination eliminated with plasmocin; negative, HOECHST and PCR
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: ECACC: 91091816, DSMZ: ACC 141
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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