SIRC's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

SIRC (rabbit, cornea)

SPECIES: rabbit
TISSUE/ORGAN: cornea

ECACC catalog code: 89090404
Morphology: Epithelial
Susceptibility to viruses: N- and B-tropic oncornaviruses C * rubella
Description: Rabbit Cornea
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Rubella virus

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin 0.02% EDTA; 5% CO2, 37C.
Karyotype: 2n = 44
Derived from the cornea of a normal rabbit. The line is suitable for primary isolation of rubella virus in addition to propagation and assay. The cells are not susceptible to poliovirus 2, coxsackie A9 and B5.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 60, IZSBS: BS CL79, ICLC: AL96001

Bibliographic references:

Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
Science 1965;149:633 - PMID: 4378694
Virology 1984;132:79-84 - PMID: 6607566

SIRC (rabbit, cornea)

IZSBS catalog code: BS CL79
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rabbit Karyology: modal number 66
TISSUE/ORGAN: cornea
Susceptibility to viruses: N- and B-tropic oncornaviruses C * rubella
PROPERTIES: virus replication; virology

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS + NaHCO3 (0,85 gr/lt); Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 440 , mycoplasma negative, culture

Availability in cell line catalogues: ATCC: CCL 60, ECACC: 89090404, ICLC: AL96001

SIRC (rabbit, cornea)

Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rabbit Karyology: modal number 66
TISSUE/ORGAN: cornea
Susceptibility to viruses: N- and B-tropic oncornaviruses C * rubella
PROPERTIES: virus replication; virology

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)

Availability in cell line catalogues: ATCC: CCL 60, IZSBS: BS CL79, ECACC: 89090404, ICLC: AL96001

Bibliographic references:

Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
Science 1965;149:633 - PMID: 4378694
Virology 1984;132:79-84 - PMID: 6607566

SIRC (rabbit, cornea)

Available in the following LABORATORY:

Universita' degli Studi (PRUMB, Parma)
MEM (HBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 20% FBS + 10% DMSO; 37C, 5% CO2 Passage: 450

Availability in cell line catalogues: ATCC: CCL 60, IZSBS: BS CL79, ECACC: 89090404, ICLC: AL96001

Bibliographic references:

Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
Science 1965;149:633 - PMID: 4378694
Virology 1984;132:79-84 - PMID: 6607566

SIRC (rabbit, cornea)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CCL 60, IZSBS: BS CL79, ECACC: 89090404, ICLC: AL96001

Bibliographic references:

Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
Science 1965;149:633 - PMID: 4378694
Virology 1984;132:79-84 - PMID: 6607566

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105