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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

SIRC (rabbit, cornea)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin 0.02% EDTA; 5% CO2, 37C.
  Karyotype: 2n = 44
  Derived from the cornea of a normal rabbit. The line is suitable for primary isolation of rubella virus in addition to propagation and assay. The cells are not susceptible to poliovirus 2, coxsackie A9 and B5.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CCL 60, IZSBS: BS CL79, ICLC: AL96001

Bibliographic references:

• Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
  
• Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
  
• Science 1965;149:633 - PMID: 4378694
  
• Virology 1984;132:79-84 - PMID: 6607566
  

SIRC (rabbit, cornea)

Available in the following LABORATORY:

• IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
  MEM (EBSS) + 10% FBS + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:4-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. Passage: 12 , mycoplasma negative, HOECHST and PCR
  Validated by: isoenzymes (confirmed as rabbit with AST, G6PD, PepB)
  

Availability in cell line catalogues: IZSBS: BS CL79, ECACC: 89090404

Bibliographic references:

• Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
• Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
• Proc Soc Exp Biol Med 1970;135:1543-1545
• Virology 1984;132:79-84 - PMID: 6607566

SIRC (rabbit, cornea)

Available in the following LABORATORY:

• Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
  MEM (EBSS) + 10% FBS + NaHCO3 (0,85 gr/lt); Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 440 , mycoplasma negative, culture
  

Availability in cell line catalogues: ATCC: CCL 60, ECACC: 89090404, ICLC: AL96001

SIRC (rabbit, cornea)

Available in the following LABORATORY:

• Universita' degli Studi (PRUMB, Parma)
  MEM (HBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 20% FBS + 10% DMSO; 37C, 5% CO2 Passage: 450
  

Availability in cell line catalogues: ATCC: CCL 60, IZSBS: BS CL79, ECACC: 89090404, ICLC: AL96001

Bibliographic references:

• Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
• Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
• Science 1965;149:633 - PMID: 4378694
• Virology 1984;132:79-84 - PMID: 6607566

SIRC (rabbit, cornea)

Available in the following LABORATORY:

• Russian Academy of Sciences (SPBIC, St. Petersburg)
  
  

Availability in cell line catalogues: ATCC: CCL 60, IZSBS: BS CL79, ECACC: 89090404, ICLC: AL96001

Bibliographic references:

• Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
• Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
• Science 1965;149:633 - PMID: 4378694
• Virology 1984;132:79-84 - PMID: 6607566