SPECIES: rabbit
TISSUE/ORGAN: cornea
ECACC catalog code: 89090404
Morphology: Epithelial
Susceptibility to viruses: N- and B-tropic oncornaviruses C * rubella
Description: Rabbit Cornea
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Rubella virus
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin 0.02% EDTA; 5% CO2, 37C.
Karyotype: 2n = 44
Derived from the cornea of a normal rabbit. The line is suitable for primary isolation of rubella virus in addition to propagation and assay. The cells are not susceptible to poliovirus 2, coxsackie A9 and B5.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 60, IZSBS: BS CL79, ICLC: AL96001
Bibliographic references:
- Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
- Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
- Science 1965;149:633 - PMID: 4378694
- Virology 1984;132:79-84 - PMID: 6607566
ICLC catalog code: AL96001
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rabbit Karyology: modal number 66
TISSUE/ORGAN: cornea
Susceptibility to viruses: rubella
DEPOSITOR: Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: virus replication; not susceptible to poliovirus2, Coxsackie A9 and B5
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:4-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. Passage: 12 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as rabbit with AST, G6PD, PepB)
Availability in cell line catalogues: IZSBS: BS CL79, ECACC: 89090404
Bibliographic references:
- Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
- Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
- Proc Soc Exp Biol Med 1970;135:1543-1545
- Virology 1984;132:79-84 - PMID: 6607566
IZSBS catalog code: BS CL79
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rabbit Karyology: modal number 66
TISSUE/ORGAN: cornea
Susceptibility to viruses: N- and B-tropic oncornaviruses C * rubella
PROPERTIES: virus replication; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 60, ECACC: 89090404, ICLC: AL96001
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rabbit Karyology: modal number 66
TISSUE/ORGAN: cornea
Susceptibility to viruses: N- and B-tropic oncornaviruses C * rubella
PROPERTIES: virus replication; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 60, IZSBS: BS CL79, ECACC: 89090404, ICLC: AL96001
Bibliographic references:
- Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
- Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
- Science 1965;149:633 - PMID: 4378694
- Virology 1984;132:79-84 - PMID: 6607566
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rabbit Karyology: modal number 66
TISSUE/ORGAN: cornea
Susceptibility to viruses: N- and B-tropic oncornaviruses C * rubella
PROPERTIES: virus replication; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 60, IZSBS: BS CL79, ECACC: 89090404, ICLC: AL96001
Bibliographic references:
- Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
- Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
- Science 1965;149:633 - PMID: 4378694
- Virology 1984;132:79-84 - PMID: 6607566
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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