SHM-D33's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

SHM-D33 (mouse / human, hybrid, heteromyeloma)

ICLC catalog code: ATL94001
Continuous culture, grown as semiadherent
lymphoblast morphology
SPECIES: mouse / human Karyology: 83 murine chromosomes + 4 human chromosomes
TISSUE/ORGAN: hybrid
TUMOR: heteromyeloma
DEPOSITOR: Dr V. Dessi, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: resistant to ouabain; fusion partner; hybridization; tumorigenicity

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: MEM + 20% FBS + 10% DMSO; maintain cultures between 3 and 10x105 cells/ml; split 1:5-1:10; the cell line grows semiadherent.37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (pattern compatible with mouse (Pep B, AST, NP))

Bibliographic references:

Proc Natl Acad Sci U S A 1983;80:7308 - PMID: 6316357

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105