SPECIES: human
TUMOR: neuroblastoma
DSMZ catalog code: ACC 209
Morphology: epithelial-like cells growing as monolayer and in cell clusters (when cultured at high density); cells do not grow to complete confluency
Description: human neuroblastoma clonal subline of the neuroepithelioma cell line SK-N-SH that had been established in 1970 from the bone marrow biopsy of a 4-year-old girl with metastatic neuroblastoma confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Heikkilae, University of Turku, Turku, Finland
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-85% Dulbecco's MEM + 15-20% FBS split culture 1-2 times a week using trypsin/EDTA; seed out with 2 x 106 cells/80 cm 2 at 37C with 5-10% CO2 cell harvest of about 15 x 106 cells/175 cm 2;
doubling time of >55 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human near-diploid karyotype with 1.8% polyploidy; 46/47(42-48)<2n>X/XX, +7, ins(1) (q32q11q43), add(9)(q34), der(22)t(?17;22)(q22;q13)) * isoenzymes (confirmed as human with IEF of AST, MDH)
Availability in cell line catalogues: ECACC: 94030304, ICLC: HTL95013
Bibliographic references:
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- Cancer Res 1978;38:3751-3757 - PMID: 29704
- Cancer Res 1990;50:3422-3428 - PMID: 2334938
SPECIES: human, Caucasian
TISSUE/ORGAN: bone marrow
TUMOR: neuroblastoma
ECACC catalog code: 94030304
Morphology: Neuroblast
Description: Human neuroblastoma
Viruses: See Properties
Depositor: Dr PFT Vaughan, Institute for Cardiovascular Research, University of Leeds, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Neurotransmitter studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12:EMEM (EBSS) (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 to 1:100 i.e. seeding at 1x1,000-1x10,000 cells/cm2 every 2-4 weeks; 5% CO2; 37C. Cells reattach slowly and may remain in suspension for several days. NB: do not use after passage 20.
Passages: 12;
SH-SY5Y is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH (ECACC No. 86012802). SH-SY-5Y has dopamine-beta-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. Will form tumours in nude mice in approximately 3-4 weeks.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: DSMZ: ACC 209, ICLC: HTL95013
Bibliographic references:
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- Cancer Res 1978;38:3751-3757 - PMID: 29704
- Cancer Res 1990;50:3422-3428 - PMID: 2334938
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 4 years old)
TISSUE/ORGAN: bone marrow
TUMOR: neuroblastoma
PROPERTIES: tumor markers; chemotherapeutic agents; nucleic acids; neurotransmitter studies; convert glutamate to GABA; dopamine-beta-hydroxylase activity
Available in the following LABORATORY:
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ENEA, CRE-Casaccia (RMEBA, Roma)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 50% FBS + 10% DMSO; 37C, 5% CO2, split 5-10.
Availability in cell line catalogues: ECACC: 94030304, DSMZ: ACC 209, ICLC: HTL95013
Bibliographic references:
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- Cancer Res 1978;38:3751-3757 - PMID: 29704
- Cancer Res 1990;50:3422-3428 - PMID: 2334938
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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