SAOS-2's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

SAOS-2 (human, Caucasian, bone, osteosarcoma)

ICLC catalog code: HTL01001
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (female, 11 years old)
TISSUE/ORGAN: bone
TUMOR: osteosarcoma
DEPOSITOR: Dr G. Di Domenico, Biology and Cellular and Molecular Pathology Dept, Naples, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: epidermal growth factor (EGF) and transforming growth factor beta (type 1 and type 2) receptor expression; positive for alkaline phosphatase

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA, 37C, 5% CO2 Passage: 15 , mycoplasma negative, HOECHST and culture
Validated by: HLA-DP beta (confirmed as human by PCR) * identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X; vWA: 18; FGA: 22,25; TH01: 6,9; D18S51: 15; D21S11: 28,30; D8S1179: 10,12)

Availability in cell line catalogues: IZSBS: BS TLC 90, DSMZ: ACC 243, ECACC: 89050205

Bibliographic references:

Cancer Res. 1987;47:4961-4966 - PMID: 3040234
Human tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 77097006
J Natl Cancer Inst 1977;59:221-226 - PMID: 77210034

SAOS-2 (human, Caucasian, bone, osteosarcoma)

IZSBS catalog code: BS TLC 90
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 11 years old) Karyology: 2n = 46 in 50 cells
TISSUE/ORGAN: bone
TUMOR: osteosarcoma
DEPOSITOR: obtained from ATCC

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
McCoy's 5A + 15% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 29 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, MD, PEPB)

Availability in cell line catalogues: ATCC: HTB 85, DSMZ: ACC 243, ICLC: HTL01001, ECACC: 89050205

Bibliographic references:

J Natl Cancer Inst 1977;58:209-214 - PMID: 833871

SAOS-2 (human, Caucasian, bone, osteosarcoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 11 years old)
TISSUE/ORGAN: bone
TUMOR: osteosarcoma

Available in the following LABORATORY:

Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISB, Milano)
RPMI 1640 + 15% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-3

Availability in cell line catalogues: ATCC: HTB 85, IZSBS: BS TLC 90, DSMZ: ACC 243, ICLC: HTL01001, ECACC: 89050205

Bibliographic references:

Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105