SPECIES: human, Caucasian
TISSUE/ORGAN: bone
TUMOR: sarcoma, osteogenic
DSMZ catalog code: ACC 243
Morphology: adherent, epithelial-like cells growing in monolayers
Description: human osteogenic sarcoma established from the primary osteogenic sarcoma of an 11-year-old Caucasian woman in 1973 (ATCC HTB 85) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85% McCoy's 5A + 15% FBS split confluent culture 1:2 to 1:3 every 2-3 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm 2 in 10 ml medium at 37 C with 5% CO2 cell harvest of about 10 x 106 cells/175 cm 2;
doubling time of about 43 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human karyotype) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin -, desmin -, endothel -, GFAP -, neurofilament -, vimentin + (100%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HTB 85, IZSBS: BS TLC 90, ICLC: HTL01001, ECACC: 89050205
Bibliographic references:
- Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
ICLC catalog code: HTL01001
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (female, 11 years old)
TISSUE/ORGAN: bone
TUMOR: osteosarcoma
DEPOSITOR: Dr G. Di Domenico, Biology and Cellular and Molecular Pathology Dept, Naples, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: epidermal growth factor (EGF) and transforming growth factor beta (type 1 and type 2) receptor expression; positive for alkaline phosphatase
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA, 37C, 5% CO2 Passage: 15 , mycoplasma negative, HOECHST and culture
Validated by: HLA-DP beta (confirmed as human by PCR) * identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X; vWA: 18; FGA: 22,25; TH01: 6,9; D18S51: 15; D21S11: 28,30; D8S1179: 10,12)
Availability in cell line catalogues: IZSBS: BS TLC 90, DSMZ: ACC 243, ECACC: 89050205
Bibliographic references:
- Cancer Res. 1987;47:4961-4966 - PMID: 3040234
- Human tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159
- J Natl Cancer Inst 1977;58:209-214 - PMID: 77097006
- J Natl Cancer Inst 1977;59:221-226 - PMID: 77210034
IZSBS catalog code: BS TLC 90
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 11 years old) Karyology: 2n = 46 in 50 cells
TISSUE/ORGAN: bone
TUMOR: osteosarcoma
DEPOSITOR: obtained from ATCC
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 85, DSMZ: ACC 243, ICLC: HTL01001, ECACC: 89050205
Bibliographic references:
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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