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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

RTG-2 (fish - trout, rainbow, gonad)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Passage using 0.25% trypsin below 24C. Cells may be seeded as few as 3x10,000 cells/ml 5% CO2; 4-24C.
  Passages: 8; Karyotype: 2n = 60
  From pooled male/female gonad tissue of yearling rainbow trout. First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24C. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CCL 55, DSMZ: ACC 352

Bibliographic references:

• Proc Soc Exp Biol Med 1964;116:190
  
• Science 1962;135:1065