RPMI 8226's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

RPMI 8226 (human, peripheral blood, myeloma)

SPECIES: human
TISSUE/ORGAN: peripheral blood
TUMOR: myeloma

ECACC catalog code: 87012702
Morphology: Suspension
Description: Human myeloma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin G (Ig) lambda light chain Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 155, IZSBS: BS TCL 92

Bibliographic references:

Proc Soc Exp Biol Med 1967;125:1246 - PMID: 6042436

RPMI 8226 (human, peripheral blood, myeloma)

IZSBS catalog code: BS TCL 92
Continuous culture, grown as suspension
Lymphoblast-like morphology
SPECIES: human Karyology: 2n = 46 in 55 cells
TISSUE/ORGAN: peripheral blood
TUMOR: myeloma
Susceptibility to viruses: herpes simplex * poliovirus 1 * vaccinia * vesic. stomatitis (Indiana)
DEPOSITOR: obtained from ATCC
PROPERTIES: used in immunoglobulin synthesis when cultivated in a chemically defined medium

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, PEPB, AST)

Availability in cell line catalogues: ATCC: CCL-155, ECACC: 87012702

Bibliographic references:

Proc Soc Exp Biol Med 1967;125:1246 - PMID: 6042436

RPMI 8226 (human, peripheral blood, myeloma)

Monoclonal continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 61 years old)
TISSUE/ORGAN: peripheral blood
TUMOR: myeloma
PROPERTIES: Ig lambda light chain secr.; Ig prod.; tumorigenicity

Available in the following LABORATORY:

Centro Trapianto Midollo Osseo e Divisione Ematologia (PSHTM, Pesaro)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + Antibiotics + 1mM Na pyruvate; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2

Availability in cell line catalogues: ATCC: CCL 155, ECACC: 87012702, IZSBS: BS TCL 92

Bibliographic references:

Proc Soc Exp Biol Med 1967;125:1246 - PMID: 6042436

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105