SPECIES: rabbit
TISSUE/ORGAN: kidney
ECACC catalog code: 88062427
Morphology: Epithelial
Susceptibility to viruses: equine viral arteritis virus * herpes B * herpes simplex * infectious bovine rhinotracheitis * myxoma * pseudorabies * rabbit myxoma virus * rabbit pox * rubella * simian adenovirus * vaccinia * vesicular stomatitis
Description: Rabbit kidney
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Expression of Cytopathic effect. Applications: Virus studies: HSV, PRV, Vaccinia, Rabbit pox, myxoma and Simian adenovirus, rubella
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 44
Derived from kidney cells of a 5 week old rabbit. Susceptible to HSV, PRV, vaccinia, rabbit pox, myxoma and Simian adenovirus. Sensitive to rubella virus and produces cytopathic effect. This cell line is positive for BVDV.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 37
Bibliographic references:
- Annali Sclavo 1982;24:336 - PMID: 6314916
- J Pathol Bacteriol 1968;95:377 - PMID: 4297026
- Lancet 1963;2:640
- Science 1965;149:633 - PMID: 4378694
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: rabbit (5 weeks old)
TISSUE/ORGAN: kidney
non tumorigenic
Susceptibility to viruses: equine viral arteritis virus * herpes B * herpes simplex * infectious bovine rhinotracheitis * myxoma * pseudorabies * rabbit myxoma virus * rabbit pox * rubella * simian adenovirus * vaccinia * vesicular stomatitis
PROPERTIES: virus prod.; cytopathic effects (CPE); virology; virus titration
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 37, ECACC: 88062427
Bibliographic references:
- Annali Sclavo 1982;24:336 - PMID: 6314916
- J Pathol Bacteriol 1968;95:377 - PMID: 4297026
- Lancet 1963;2:640
- Science 1965;149:633 - PMID: 4378694
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: rabbit (5 weeks old)
TISSUE/ORGAN: kidney
non tumorigenic
Susceptibility to viruses: equine viral arteritis virus * herpes B * herpes simplex * infectious bovine rhinotracheitis * myxoma * pseudorabies * rabbit myxoma virus * rabbit pox * rubella * simian adenovirus * vaccinia * vesicular stomatitis
PROPERTIES: virus prod.; cytopathic effects (CPE); virology; virus titration
Available in the following LABORATORY:
-
Universita' degli Studi (PRUMB, Parma)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: MEM + 20% FBS + 10% DMSO; 37C, 5% CO2 Passage: 77
Availability in cell line catalogues: ATCC: CCL 37, ECACC: 88062427
Bibliographic references:
- Annali Sclavo 1982;24:336 - PMID: 6314916
- J Pathol Bacteriol 1968;95:377 - PMID: 4297026
- Lancet 1963;2:640
- Science 1965;149:633 - PMID: 4378694
Monoclonal continuous culture, grown as monolayer
epithelial morphology
SPECIES: rabbit (5 weeks old)
TISSUE/ORGAN: kidney
non tumorigenic
Susceptibility to viruses: equine viral arteritis virus * herpes B * herpes simplex * infectious bovine rhinotracheitis * myxoma * pseudorabies * rabbit myxoma virus * rabbit pox * rubella * simian adenovirus * vaccinia * vesicular stomatitis
PROPERTIES: virus prod.; cytopathic effects (CPE); virology; virus titration
Available in the following LABORATORY:
-
Dip. Biologia Molecolare, Universita' degli Studi (SIUVI, Siena)
MEM modified + 5% FBS + Antibiotics + NaHCO3; Freezing medium: MEM modified + 20% FBS + 7.5% DMSO; 37C, 5% CO2, split 1-5 Passage: 200 , mycoplasma negative, enzyme immunoassay (EIA)
Availability in cell line catalogues: ATCC: CCL 37, ECACC: 88062427
Bibliographic references:
- Annali Sclavo 1982;24:336 - PMID: 6314916
- J Pathol Bacteriol 1968;95:377 - PMID: 4297026
- Lancet 1963;2:640
- Science 1965;149:633 - PMID: 4378694
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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