RD's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

RD (human, Caucasian, muscle, rhabdomyosarcoma, embryonal)

Continuous culture, grown as monolayer
spindle-shaped morphology
SPECIES: human, Caucasian (female, 7 years old) Karyology: modal number 50;microchromosomes, chr. association
TISSUE/ORGAN: muscle
TUMOR: rhabdomyosarcoma, embryonal
Susceptibility to viruses: Coxsackie A * enterovirus * herpes simplex 1 * poliovirus 1 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: protein secr.; myoglobin ATPase activity; myosin ATPase activity; genetics; nucleic acids; tumorigenicity; differentiation; cell biology; protein

Available in the following LABORATORY:

Dip. Scienze Biomediche e Biotecnologie, Universita' degli Studi (BGEBS, Brescia)
MEM + 10% FBS; Freezing medium: MEM + 30% FBS + 10% DMSO; 37C, 5% CO2

Availability in cell line catalogues: ATCC: CCL 136, ECACC: 85111502

Bibliographic references:

Cancer 1969;24:520 - PMID: 4241949
J Clin Microbiol 1990;28:1049 - PMID: 2161864
J Virol 1967;1:326

RD (human, Caucasian, muscle, rhabdomyosarcoma, embryonal)

Available in the following LABORATORY:

Clinica Pediatrica, Ospedale S.Orsola-Malpighi (BOHIC, Bologna)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1% Antibiotics + 1% non-essential amino acids; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-4 , mycoplasma negative, DAPI and HOECHST
Cloning efficiency <1%. Split twice a week
Validated by: membrane markers

Availability in cell line catalogues: ATCC: CCL 136, ECACC: 85111502

Bibliographic references:

Cancer 1969;24:520 - PMID: 4241949
J Clin Microbiol 1990;28:1049 - PMID: 2161864
J Virol 1967;1:326

RD (human, Caucasian, muscle, rhabdomyosarcoma, embryonal)

Continuous culture, grown as monolayer
spindle-shaped morphology
SPECIES: human, Caucasian (female, 7 years old) Karyology: modal number 50;microchromosomes, chr. association
TISSUE/ORGAN: muscle
TUMOR: rhabdomyosarcoma, embryonal
Susceptibility to viruses: Coxsackie A * enterovirus * herpes simplex 1 * poliovirus 1 * vaccinia * vesic. stomatitis (Indiana)
Cell line available under some conditions.
PROPERTIES: protein secr.; myoglobin ATPase activity; myosin ATPase activity; genetics; nucleic acids; tumorigenicity; differentiation; cell biology; protein

Available in the following LABORATORY:

Istituto di Cancerologia, Universita' degli Studi (BOUCN, Bologna)
DMEM + 10% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 7% CO2, split 4-8 Passage: 57 , mycoplasma negative, HOECHST
Morphology is spindle-shaped, myotube-like, giant cells

Availability in cell line catalogues: ATCC: CCL 136, ECACC: 85111502

Bibliographic references:

Cancer 1969;24:520 - PMID: 4241949
J Clin Microbiol 1990;28:1049 - PMID: 2161864
J Virol 1967;1:326

RD (human, Caucasian, muscle, rhabdomyosarcoma, embryonal)

SPECIES: human, Caucasian
TISSUE/ORGAN: muscle
TUMOR: rhabdomyosarcoma, embryonal

ECACC catalog code: 85111502
Morphology: Spindle-shaped
Susceptibility to viruses: Coxsackie A * enterovirus * herpes simplex 1 * poliovirus 1 * vaccinia * vesic. stomatitis (Indiana)
Description: Human Caucasian embryo rhabdomyosarcoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Passages: 40; Karyotype: Modal No 56
Derived from a malignant embryonal rhabdomyosarcoma from a 7 year old Caucasian female. Also designated TE 32 and 130T in the literature
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 136

Bibliographic references:

Cancer 1969;24:520 - PMID: 4241949
J Clin Microbiol 1990;28:1049 - PMID: 2161864
J Virol 1967;1:326

RD (human, Caucasian, muscle, rhabdomyosarcoma, embryonal)

Available in the following LABORATORY:

Universita' degli Studi (PGUPG, Perugia)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% Glycerol; 37C, 5% CO2 Passage: 71

Availability in cell line catalogues: ATCC: CCL 136, ECACC: 85111502

Bibliographic references:

Cancer 1969;24:520 - PMID: 4241949
J Clin Microbiol 1990;28:1049 - PMID: 2161864
J Virol 1967;1:326

RD (human, Caucasian, muscle, rhabdomyosarcoma, embryonal)

Available in the following LABORATORY:

Universita' La Sapienza (RMUPG, Roma)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 5% CO2

Availability in cell line catalogues: ATCC: CCL 136, ECACC: 85111502

Bibliographic references:

Cancer 1969;24:520 - PMID: 4241949
J Clin Microbiol 1990;28:1049 - PMID: 2161864
J Virol 1967;1:326

RD (human, Caucasian, muscle, rhabdomyosarcoma, embryonal)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CCL 136, ECACC: 85111502

Bibliographic references:

Cancer 1969;24:520 - PMID: 4241949
J Clin Microbiol 1990;28:1049 - PMID: 2161864
J Virol 1967;1:326

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105