SPECIES: rat
TUMOR: leukemia, basophilic
ECACC catalog code: 86061001
Morphology: Lymphoblast
Description: Rat basophilic leukaemia granulocyte
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10-20% Fetal Bovine Serum (FBS). Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 5% CO2; 37C.
Exhibits marked variations in differentiation when monitored for basophilic granule development, histamine content and membrane receptor sites for IgE.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1378, DSMZ: ACC 147, ICLC: ATL01001
Bibliographic references:
- J Exp Med 1974;139:600-616 - PMID: 4812630
- Nature New Biol 1973;244:73-75 - PMID: 4516143
ICLC catalog code: ATL01001
Continuous culture, grown as monolayer
epithelial-like and some round cells morphology
SPECIES: rat, Wistar
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, basophilic
DEPOSITOR: Dr A. Rubartelli, IST, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
-
IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:5-1:10 using trypsin/EDTA; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Availability in cell line catalogues: ECACC: 86061001, DSMZ: ACC 147
Bibliographic references:
- J. Exp. Med. 1974;139:600-616 - PMID: 4812630
- Nature New Biology 1973;244:73-76 - PMID: 4516144
Continuous culture, grown as suspension
lymphoblast morphology
SPECIES: rat
TUMOR: leukemia, basophilic
PROPERTIES: differentiation
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1378, ECACC: 86061001, DSMZ: ACC 147, ICLC: ATL01001
Bibliographic references:
- J Exp Med 1974;139:600-616 - PMID: 4812630
- Nature New Biol 1973;244:73-75 - PMID: 4516143
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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