SPECIES: rat
TUMOR: leukemia, basophilic
DSMZ catalog code: ACC 147
Morphology: 1/4 adherent (epithelial-like cells), 3/4 in suspension (small round cells)
Description: rat basophilic leukemia this rat basophilic leukemia (RBL) cell line was established from chlorethylamine-treated rats; the cells were described to show marked variations in differentiation dependent on the growth rate and the cell cycle phases confirmed as rat with IEF of AST, NP
Viruses: ELISA. reverse transcriptase negative
Depositor: obtained from ATCC (CRL 1378), Rockville, Maryland, USA
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS (cells may also be grown, but less efficiently, with MEM) maximal split ratio of 1:4; cells grow partly adherent, but trypsin is not needed to detach the cells at 37C with 5% CO2 about 46 x 106 cells/175 cm 2;
doubling time of ca. 25 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (74(69-76); rodent marker chromosomes present) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as rat with IEF of AST, NP)
Availability in cell line catalogues: ATCC: CRL 1378, ECACC: 86061001, ICLC: ATL01001
Bibliographic references:
- J Exp Med 1974;139:600-616 - PMID: 4812630
- Nature New Biol 1973;244:73-75 - PMID: 4516143
ICLC catalog code: ATL01001
Continuous culture, grown as monolayer
epithelial-like and some round cells morphology
SPECIES: rat, Wistar
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, basophilic
DEPOSITOR: Dr A. Rubartelli, IST, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:5-1:10 using trypsin/EDTA; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Availability in cell line catalogues: ECACC: 86061001, DSMZ: ACC 147
Bibliographic references:
- J. Exp. Med. 1974;139:600-616 - PMID: 4812630
- Nature New Biology 1973;244:73-76 - PMID: 4516144
Continuous culture, grown as suspension
lymphoblast morphology
SPECIES: rat
TUMOR: leukemia, basophilic
PROPERTIES: differentiation
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1378, ECACC: 86061001, DSMZ: ACC 147, ICLC: ATL01001
Bibliographic references:
- J Exp Med 1974;139:600-616 - PMID: 4812630
- Nature New Biol 1973;244:73-75 - PMID: 4516143
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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