RAJI's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

RAJI (human, Black, lymphoma, Burkitt)

ICLC catalog code: HTL00002
Continuous culture, grown as suspension
lymphoblast-like, round cells, single and in clumps morphology
SPECIES: human, Black (male, 11 years old)
TUMOR: lymphoma, Burkitt
DEPOSITOR: Prof. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: EBNA positive

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 0.4-1 x 10^6 cells/ml; split 1:5-1:10; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
After thawing the cells look suffering; thaw with medium supplemented with 20% FBS
Validated by: HLA-DP beta (confirmed as human by PCR) * identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X,Y; vWA: 16,19,18; FGA: 19,27; TH01: 6,7; D18S51: 17; D21S11: 28,31; D8S1179: 14,15)

Availability in cell line catalogues: IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319

Bibliographic references:

J Exp Med 1976;143:1528-1533 - PMID: 1083890
J Natl Cancer Inst 1966;37:547 - PMID: 4288580
Lancet 1964;1:238-240
Trans N Y Acad Sci 1966;29:71 - PMID: 5232223

RAJI (human, Black, lymphoma, Burkitt)

IZSBS catalog code: BS TCL54
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 11 years old)
TUMOR: lymphoma, Burkitt
Susceptibility to viruses: arbovirus * poliovirus * simian retrovirus, type D * vesicular stomatitis
PROPERTIES: B cell differentiation; immunology; antitumour testing; virology

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
RPMI 1640 + 20% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 120 , mycoplasma negative, culture
Obtained from American Tyoe Collection,Rockville, Md (USA).

Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, ECACC: 85011429, DSMZ: ACC 319

RAJI (human, Black, lymphoma, Burkitt)

Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 11 years old)
TUMOR: lymphoma, Burkitt
Susceptibility to viruses: arbovirus * poliovirus * simian retrovirus, type D * vesicular stomatitis
PROPERTIES: B cell differentiation; immunology; antitumour testing; virology

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)
mycoplasma negative

Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319

Bibliographic references:

J Clin Pathol 1965;18:261
J Natl Cancer Inst 1966;37:547 - PMID: 4288580
Lancet 1964;1:238
Trans N Y Acad Sci 1966;29:61

RAJI (human, Black, lymphoma, Burkitt)

Monoclonal continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 11 years old)
TUMOR: lymphoma, Burkitt
Susceptibility to viruses: arbovirus * poliovirus * simian retrovirus, type D * vesicular stomatitis
PROPERTIES: B cell differentiation; immunology; antitumour testing; virology

Available in the following LABORATORY:

Centro Trapianto Midollo Osseo e Divisione Ematologia (PSHTM, Pesaro)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + Antibiotics + 1mM Na pyruvate; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2

Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319

Bibliographic references:

J Clin Pathol 1965;18:261
J Natl Cancer Inst 1966;37:547 - PMID: 4288580
Lancet 1964;1:238
Trans N Y Acad Sci 1966;29:61

RAJI (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

Istituto Superiore di Sanità (RMSVI, Roma)
RPMI 1640 + 15% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-6

Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319

Bibliographic references:

J Clin Pathol 1965;18:261
J Natl Cancer Inst 1966;37:547 - PMID: 4288580
Lancet 1964;1:238
Trans N Y Acad Sci 1966;29:61

RAJI (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Positive for Epstein-Barr nuclear antigen, but does not contain the EB virus

Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319

Bibliographic references:

J Clin Pathol 1965;18:261
J Natl Cancer Inst 1966;37:547 - PMID: 4288580
Lancet 1964;1:238
Trans N Y Acad Sci 1966;29:61

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105