SPECIES: human, Black
TUMOR: lymphoma, Burkitt
ECACC catalog code: 85011429
Morphology: Lymphoblastoid
Susceptibility to viruses: arbovirus * poliovirus * simian retrovirus, type D * vesicular stomatitis
Description: Human Negroid Burkitt's lymphoma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Resistance and virus studies: VSV
Available in the following LABORATORY:
-
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Karyotype: 2n = 46, diploid
Established in 1963 from Burkitt's lymphoma in an 11 year old Negro male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, DSMZ: ACC 319
Bibliographic references:
- J Clin Pathol 1965;18:261
- J Natl Cancer Inst 1966;37:547 - PMID: 4288580
- Lancet 1964;1:238
- Trans N Y Acad Sci 1966;29:61
ICLC catalog code: HTL00002
Continuous culture, grown as suspension
lymphoblast-like, round cells, single and in clumps morphology
SPECIES: human, Black (male, 11 years old)
TUMOR: lymphoma, Burkitt
DEPOSITOR: Prof. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: EBNA positive
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 0.4-1 x 10^6 cells/ml; split 1:5-1:10; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
After thawing the cells look suffering; thaw with medium supplemented with 20% FBS
Validated by: HLA-DP beta (confirmed as human by PCR) * identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X,Y; vWA: 16,19,18; FGA: 19,27; TH01: 6,7; D18S51: 17; D21S11: 28,31; D8S1179: 14,15)
Availability in cell line catalogues: IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319
Bibliographic references:
- J Exp Med 1976;143:1528-1533 - PMID: 1083890
- J Natl Cancer Inst 1966;37:547 - PMID: 4288580
- Lancet 1964;1:238-240
- Trans N Y Acad Sci 1966;29:71 - PMID: 5232223
IZSBS catalog code: BS TCL54
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 11 years old)
TUMOR: lymphoma, Burkitt
Susceptibility to viruses: arbovirus * poliovirus * simian retrovirus, type D * vesicular stomatitis
PROPERTIES: B cell differentiation; immunology; antitumour testing; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, ECACC: 85011429, DSMZ: ACC 319
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 11 years old)
TUMOR: lymphoma, Burkitt
Susceptibility to viruses: arbovirus * poliovirus * simian retrovirus, type D * vesicular stomatitis
PROPERTIES: B cell differentiation; immunology; antitumour testing; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319
Bibliographic references:
- J Clin Pathol 1965;18:261
- J Natl Cancer Inst 1966;37:547 - PMID: 4288580
- Lancet 1964;1:238
- Trans N Y Acad Sci 1966;29:61
Monoclonal continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 11 years old)
TUMOR: lymphoma, Burkitt
Susceptibility to viruses: arbovirus * poliovirus * simian retrovirus, type D * vesicular stomatitis
PROPERTIES: B cell differentiation; immunology; antitumour testing; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319
Bibliographic references:
- J Clin Pathol 1965;18:261
- J Natl Cancer Inst 1966;37:547 - PMID: 4288580
- Lancet 1964;1:238
- Trans N Y Acad Sci 1966;29:61
Monoclonal continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (male, 11 years old)
TUMOR: lymphoma, Burkitt
Susceptibility to viruses: arbovirus * poliovirus * simian retrovirus, type D * vesicular stomatitis
PROPERTIES: B cell differentiation; immunology; antitumour testing; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 86, ICLC: HTL00002, IZSBS: BS TCL54, ECACC: 85011429, DSMZ: ACC 319
Bibliographic references:
- J Clin Pathol 1965;18:261
- J Natl Cancer Inst 1966;37:547 - PMID: 4288580
- Lancet 1964;1:238
- Trans N Y Acad Sci 1966;29:61
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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