RAG's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

RAG (mouse, BALB/cd, kidney, adenocarcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: mouse, BALB/cd
TISSUE/ORGAN: kidney
TUMOR: adenocarcinoma
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
PROPERTIES: ES-2 prod.; resistant to 8-azaguanine; tumorigenicity; somatic cell hybridization

Available in the following LABORATORY:

Consorzio Mario Negri SUD, Centro di Ricerche Biomediche e Farmacologiche (CHIBC, S.Maria Imbaro (CH))
MEM + 10% FBS; Freezing medium: Culture medium + 5% Glycerol; 37C, 5% CO2 Passage: 259
Nonreverting 8-azaguanine resistant mutant

Availability in cell line catalogues: ATCC: CCL 142, ECACC: 89040605

Bibliographic references:

J Cell Biol 1970;45:74-82 - PMID: 4318843
Proc Natl Acad Sci U S A 1970;66:1220 - PMID: 4920091

RAG (mouse, BALB/cd, kidney, adenocarcinoma)

SPECIES: mouse, BALB/cd
TISSUE/ORGAN: kidney
TUMOR: adenocarcinoma

ECACC catalog code: 89040605
Morphology: Ameboid-like
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
Description: Mouse BALB/cd renal adenocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: ES-2 Applications: Hybridization, resistance and tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (HBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Passages: 268; Karyotype: Modal No 68, hypertriploid
Derived from the original Renal-2a BALB/cd renal adenocarcinoma. Cells produce the unique kidney specific esterase ES-2 and have been used in somatic cell hybridisation studies because of their azaguanine resistant properties.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 142

Bibliographic references:

J Cell Biol 1970;45:74-82 - PMID: 4318843
Proc Natl Acad Sci U S A 1970;66:1220 - PMID: 4920091

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105