PC-12's homonymous lines

Version 4.0.201701

Home - Cell Index - Search - Web Sites
Species - Tissues - Tumors - Pathologies - Tr. Agents
Collections - Laboratories - Catalogues

For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

PC-12 (rat, New England Deaconess Hospital strain, adrenal medulla, pheochromocytoma)

SPECIES: rat, New England Deaconess Hospital strain
TISSUE/ORGAN: adrenal medulla
TUMOR: pheochromocytoma

ECACC catalog code: 88022401
Morphology: With collagen - neuronal fibroblasts; without collagen - spherical clusters
Description: Rat adrenal phaeochromocytoma
Depositor: Obtained from ATCC, USA

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Horse Serum (HS) + 5% Fetal Bovine Serum (FBS). Adheres poorly to plastic; grows in small clusters. Use collagen coated flasks for attachment. Feed 3 times a week. Grows satisfactorily in suspension in untreated flasks. Maintain between 2-5x100,000 cells/ml; 5% CO2; 37C.

Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1721, DSMZ: ACC 159, ICLC: ATL98004, IZSBS: BS TCL 91

Bibliographic references:

EMBO J 1983;2:643 - PMID: 6641712
Methods Enzymol 1984;1147:207
Proc Natl Acad Sci U S A 1976;73:2424-2428 - PMID: 1065897
Science 1985;229:393-395 - PMID: 3839317

PC-12 (rat, adrenal, pheochromocytoma)

ICLC catalog code: ATL98004
Continuous culture, grown as suspension
clusters of small cells morphology
SPECIES: rat
TISSUE/ORGAN: adrenal
TUMOR: pheochromocytoma
DEPOSITOR: Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: the cells are described to synthesize catecholamines (dopamine, norepinephrine); respond reversibly to NGF by induction of the neuronal phenotype.

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI + 5% FBS + 10% HS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split 1:3-1:6; seed at 1-2 x 10^5 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
to obtain single cells, pass the suspension through a 22G syringe; use collagen-coated flasks for attachment

Availability in cell line catalogues: ECACC: 88022401, DSMZ: ACC 159, IZSBS: BS TCL 91

Bibliographic references:

Proc Natl Acad Sci USA 1976;73:2424-2426
Science 1985;229:393-395 - PMID: 3839317

PC-12 (rat, adrenal medulla, pheochromocytoma)

IZSBS catalog code: BS TCL 91
Continuous culture, grown as monolayer
Neuron-like morphology
SPECIES: rat
TISSUE/ORGAN: adrenal medulla
TUMOR: pheochromocytoma
DEPOSITOR: obtained from ATCC
PROPERTIES: these cells may be useful model systems for neurobiological and neurochemical studies; expressing xenobiotic metabolizing enzymes; respond reversibly to nerve growth factor

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
RPMI 1640 + 10% FBS + 15% HS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as rat with NP, G6PD, LD)

Availability in cell line catalogues: ATCC: CRL-1721, ECACC: 88022401, DSMZ: ACC 159, ICLC: ATL98004

Bibliographic references:

Science 1985;229:393-395 - PMID: 3839317

PC-12 (rat, New England Deaconess Hospital strain, adrenal medulla, pheochromocytoma)

Monoclonal continuous culture, grown as monolayer
chromaffin cells morphology
SPECIES: rat, New England Deaconess Hospital strain
TISSUE/ORGAN: adrenal medulla
TUMOR: pheochromocytoma
tumorigenic in syngeneic animals
PROPERTIES: catecholamine prod.; neurotransmitter prod.; NPP prod.; dopamine prod.; differentiation; neurobiochemistry; neurobiology

Available in the following LABORATORY:

Universita' degli Studi (PGUPG, Perugia)
RPMI 1640 + 10% HS; Freezing medium: Culture medium + 10% Glycerol; 37C, 5% CO2 Passage: 20
Differentiation induced by NGF.

Availability in cell line catalogues: ATCC: CRL 1721, ECACC: 88022401, DSMZ: ACC 159, ICLC: ATL98004, IZSBS: BS TCL 91

Bibliographic references:

EMBO J 1983;2:643 - PMID: 6641712
Methods Enzymol 1984;1147:207
Proc Natl Acad Sci U S A 1976;73:2424-2428 - PMID: 1065897
Science 1985;229:393-395 - PMID: 3839317

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105