PA-1's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

PA-1 (human, Caucasian, ovary, teratocarcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: ovary
TUMOR: teratocarcinoma

ECACC catalog code: 90013101
Morphology: Epithelial
Description: Human Caucasian ovary teratocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS) (Heat Inactivated). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 340; Karyotype: 2n = 46, pseudodiploid
Derived from ascitic fluid cells of a 12 year old Caucasian female with teratocarcinoma of the ovary. The cells produce tumours in nude mice.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1572, ICLC: HTL97002

Bibliographic references:

In Vitro 1974;10:382
Int J Cancer 1980;25:19-32
J Natl Cancer Inst 1974;52:921 - PMID: 4524119

PA-1 (human, Caucasian, ovary, teratocarcinoma)

Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 12 years old)
TISSUE/ORGAN: ovary
TUMOR: teratocarcinoma
tumorigenic in nude mice
PROPERTIES: chemoinvasion; matrigel invasion; chemotaxis; tumorigenicity

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro (GEICH, Genova)
DMEM + 10% FBS; Freezing medium: DMEM + 20% FBS + 10% DMSO; 37C, 5% CO2, split 2-10 Passage: 350
Cells derived from ascitic fluid

Availability in cell line catalogues: ATCC: CRL 1572, ECACC: 90013101, ICLC: HTL97002

Bibliographic references:

In Vitro 1974;10:382
Int J Cancer 1980;25:19-32
J Natl Cancer Inst 1974;52:921 - PMID: 4524119

PA-1 (human, Caucasian, ovary, teratocarcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 12 years old)
TISSUE/ORGAN: ovary
TUMOR: teratocarcinoma
tumorigenic in nude mice
PROPERTIES: chemoinvasion; matrigel invasion; chemotaxis; tumorigenicity

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CRL 1572, ECACC: 90013101, ICLC: HTL97002

Bibliographic references:

In Vitro 1974;10:382
Int J Cancer 1980;25:19-32
J Natl Cancer Inst 1974;52:921 - PMID: 4524119

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105