P3X63Ag8U.1's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

P3X63Ag8U.1 (mouse, BALB/c, plasmacytoma)

Monoclonal continuous culture, grown as suspension
lymphoid morphology
SPECIES: mouse, BALB/c
TUMOR: plasmacytoma
PROPERTIES: antibody prod.; fusion partner; protein; biochemistry

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro (GEIAP, Genova)
DMEM + 10% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-4

Availability in cell line catalogues: ATCC: CRL 1597, ECACC: 94120802, ICLC: ATL96012

Bibliographic references:

Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551
J Exp Med 1979;150:580 - PMID: 90108

P3X63Ag8U.1 (mouse, BALB/c, plasmacytoma)

Monoclonal continuous culture, grown as suspension
lymphoid morphology
SPECIES: mouse, BALB/c
TUMOR: plasmacytoma
PROPERTIES: antibody prod.; fusion partner; protein; biochemistry

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (GEIIB, Genova)
DMEM + 10% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-5
Established in Albert Einstein Institute,SHARFF NY

Availability in cell line catalogues: ATCC: CRL 1597, ECACC: 94120802, ICLC: ATL96012

Bibliographic references:

Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551
J Exp Med 1979;150:580 - PMID: 90108

P3X63Ag8U.1 (mouse, BALB/c, myeloma)

ICLC catalog code: ATL96012
Continuous culture, grown as suspension
lymphoblast morphology
SPECIES: mouse, BALB/c (female)
TUMOR: myeloma
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: fusion partner; HGPRT- and HAT sensitive; does not synthesize gamma1 chains, produces kappa light chains which are not secreted

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split 1:5-1:10; maintain cultures between 3 and 9x105 cells/ml; rapid cell death occurs at >1x106 cells/ml. 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with AST, G6PD)

Availability in cell line catalogues: ECACC: 94120802

Bibliographic references:

Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105