P3X63Ag8U.1's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

P3X63Ag8U.1 (mouse, BALB/c, plasmacytoma)

SPECIES: mouse, BALB/c
TUMOR: plasmacytoma

ECACC catalog code: 94120802
Morphology: Lymphoblast
Description: Mouse Myeloma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: This cell line is useful for fusion to spleen cells to produce hybridomas or for hybridization studies with other myelomas or lymphomas. Can also be used for immunoglobulin production.

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Maintain cultures at 5-9x100,000 cells/ml; 5% CO2: 37C.
Passages: +3; P3x63Ag8U.1 is a derivative of the P3x63Ag8 cell line (ECACC 85011401) from the mouse myeloma MOPC21, which was derived from a female BALB/c mouse. This variant no longer synthesises gamma-1 chains but still produces kappa light chains which are not secreted.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1597, ICLC: ATL96012

Bibliographic references:

Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551
J Exp Med 1979;150:580 - PMID: 90108

P3X63Ag8U.1 (mouse, BALB/c, plasmacytoma)

Monoclonal continuous culture, grown as suspension
lymphoid morphology
SPECIES: mouse, BALB/c
TUMOR: plasmacytoma
PROPERTIES: antibody prod.; fusion partner; protein; biochemistry

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro (GEIAP, Genova)
DMEM + 10% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-4

Availability in cell line catalogues: ATCC: CRL 1597, ECACC: 94120802, ICLC: ATL96012

Bibliographic references:

Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551
J Exp Med 1979;150:580 - PMID: 90108

P3X63Ag8U.1 (mouse, BALB/c, myeloma)

ICLC catalog code: ATL96012
Continuous culture, grown as suspension
lymphoblast morphology
SPECIES: mouse, BALB/c (female)
TUMOR: myeloma
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: fusion partner; HGPRT- and HAT sensitive; does not synthesize gamma1 chains, produces kappa light chains which are not secreted

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split 1:5-1:10; maintain cultures between 3 and 9x105 cells/ml; rapid cell death occurs at >1x106 cells/ml. 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with AST, G6PD)

Availability in cell line catalogues: ECACC: 94120802

Bibliographic references:

Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105