P3X63Ag8's homonymous lines

Version 4.0.201701

Home - Cell Index - Search - Web Sites
Species - Tissues - Tumors - Pathologies - Tr. Agents
Collections - Laboratories - Catalogues

For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

P3X63Ag8 (mouse, BALB/c, myeloma)

SPECIES: mouse, BALB/c
TUMOR: myeloma

ECACC catalog code: 85011401
Morphology: Lymphoblast
Description: Mouse myeloma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin G (IgG1) (kappa) Applications: Fusion partner

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM or RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 5x10,000 to 3x100,000 cells/ml; 5% CO2; 37C. Cells will achieve maximum cell density of 5x100,000 cells/ml.
Karyotype: Aneuploidy
Derived from the P3X27 cell line, itself a derivative of mineral oil-induced plasmacytoma MOPC-21 in BALB/c mice. Resistant to 20痢/ml azaguanine, does not grow in selective medium. HGPRT neg., dies in HAT. Doubling time 16-26 h.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: TIB 9, ICLC: ATL05001

Bibliographic references:

Eur J Immunol 1976;6:511-519 - PMID: 825377
Exp Cell Res 1970;60:61-77 - PMID: 5439579
Nature 1975;256:495 - PMID: 1172191
Science 1964;145:709

P3X63Ag8 (mouse, BALB/c, lymphoblast, myeloma)

ICLC catalog code: ATL05001
Continuous culture, grown as suspension
lymphoblast, round, single and some small clasters morphology
SPECIES: mouse, BALB/c
TISSUE/ORGAN: lymphoblast
TUMOR: myeloma
DEPOSITOR: Dr. R. P. Revoltella, Fondazione ONLUS - Staminali e Vita - Pisa, Italy
Cell line available for distribution. For non-commercial investigative use only

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium 50% + 40%FCS + 10%DMSO; split 1:10, 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR

Availability in cell line catalogues: ECACC: 85011401

Bibliographic references:

Nature 1975;256(5517):495-497 - PMID: 15728446

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105