P388.D1 (Clone 3124)'s homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

P388.D1 (Clone 3124) (mouse, lymphoid, lymphoid neoplasm)

ICLC catalog code: ATL97013
Continuous culture, grown as semiadherent
macrophage-like morphology
SPECIES: mouse
TISSUE/ORGAN: lymphoid
TUMOR: lymphoid neoplasm
Original line: P388
Transformed by: methylcholanthrene-induced
tumorigenic in nude mice
DEPOSITOR: Dr A. Rubartelli, Clinical Pathology Dept, IST, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: selected for the high production of IL-1; the cells exhibit high effector activity in antibody dependent cell mediated cytotoxicity

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
L-15 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6; seed at 2-4 x 10^4 cells/cm2; 37C , mycoplasma negative, HOECHST and PCR
the cells detach very easily; CO2 is not required
Validated by: isoenzymes (confirmed as mouse with MD, Pep B)

Availability in cell line catalogues: ECACC: 85011439

Bibliographic references:

Am J Pathol 1957;33:603
Cancer Res 1977;37:546-550 - PMID: 318922
J Immunol 1975;114:894-897 - PMID: 1167564
J Immunol 1977;119:950-954 - PMID: 894031

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105