Monoclonal continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rat
TISSUE/ORGAN: kidney
PROPERTIES: contact inhibition; transformation; chemical transformation; viral transformation
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1570, ECACC: 86101301, DSMZ: ACC 172
Bibliographic references:
- J Bacteriol 1966;92:1133-1140 - PMID: 5926742
- J Cell Physiol 1978;94:335 - PMID: 304450
SPECIES: rat
TISSUE/ORGAN: kidney, fibroblast
DSMZ catalog code: ACC 172
Morphology: fibroblast-like cells with a fusiform shape
Description: rat kidney fibroblasts cell line was cloned from a mixed culture of normal rat kidney cells; cells exhibit contact inhibition and are very sensitive; indicator cells for viral or chemical transformation; this cell line is frequently used for TGF-ss bioassays; (= ATCC CRL 1570) confirmed as rat with IEF of AST, NP, PEP B
Viruses: ELISA. reverse transcriptase negative
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
95% Dulbecco's MEM + 5% FBS split culture 1:4 to 1:5 about 2-3 times a week using trypsin/EDTA when the cells have reached 3/4 of confluence at 37C with 5% CO2 cell harvest of about 3.5 x 106 /80 cm 2;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-diploid rat karyotype with 4% polyploidy; 40(37-42)<2n>) * isoenzymes (confirmed as rat with IEF of AST, NP, PEP B)
Availability in cell line catalogues: ATCC: CRL 1570, ECACC: 86101301
Bibliographic references:
- J Bacteriol 1966;92:1133-1140 - PMID: 5926742
- J Cell Physiol 1978;94:335 - PMID: 304450
SPECIES: rat
TISSUE/ORGAN: kidney
ECACC catalog code: 86101301
Morphology: Fibroblast
Description: Rat kidney fibroblast
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Contact inhibition and transformation studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Fetal Bovine Serum (FBS) or New Born Calf Serum (NBCS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. The cells should be maintained sub-confluent, otherwise they will transform.
Passages: 29;
Derived from normal kidney and exhibits contact-inhibition. It is sensitive to viral or chemical transformation, including proteins such as SGF. Originated from the same mixed culture as NRK-52E but has distinct characteristics.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1570, DSMZ: ACC 172
Bibliographic references:
- J Bacteriol 1966;92:1133-1140 - PMID: 5926742
- J Cell Physiol 1978;94:335 - PMID: 304450
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rat
TISSUE/ORGAN: kidney
PROPERTIES: contact inhibition; transformation; chemical transformation; viral transformation
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1570, ECACC: 86101301, DSMZ: ACC 172
Bibliographic references:
- J Bacteriol 1966;92:1133-1140 - PMID: 5926742
- J Cell Physiol 1978;94:335 - PMID: 304450
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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