SPECIES: mouse, albino
TUMOR: neuroblastoma
DSMZ catalog code: ACC 148
Morphology: about 30% of the cells grow like neuronal cells; 70% are round, loosely attached cells
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
Description: mouse neuroblastoma established from the spontaneous tumor of a strain A albino mouse confirmed as mouse with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: obtained from ATCC (CCL 131), Rockville, Maryland, USA
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS + 1x non-essential amino acids split culture 1:4 about once a week using trypsin/EDTA, cells do not grow to confluence and grow rather slowly; seed out at ca. 1 x 106 cells/25 cm 2 at 37C with 5% CO2 about 8 x 106 cells/75 cm 2 ; maximal density at ca. 20 x 106 cells/175 cm 2;
doubling time of ca. 70 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as mouse with IEF of AST, MDH, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CCL 131, ICLC: ATL99007, IZSBS: BS TCL 128
Bibliographic references:
- J Cell Biol 1969;43:69a
- Proc Natl Acad Sci U S A 1970;65:129-136 - PMID: 5263744
ICLC catalog code: ATL99007
Monoclonal continuous culture, grown as monolayer
neuronal-like and round, mostly loosely attached cells, some in suspension morphology
SPECIES: mouse, albino
TUMOR: neuroblastoma
DEPOSITOR: Dr G. Angelini, Biotechnology Dept, IST at ABC, Genoa, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: the cells are reported to produce large quantities of microtubular protein, which plays a role in a contractile system which is responsible for axoplasmic flow in nerve cells
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split subconfluent cultures 1:3-1:6; seed at 2-4 x 104 cells/cm2; 5% CO2, 37°C
Validated by: isoenzymes (confirmed as mouse with MD, NP)
Availability in cell line catalogues: DSMZ: ACC 148, IZSBS: BS TCL 128
Bibliographic references:
- J. Cell Biol. 1969, 43:69A; Proc: Natl Acad. Sci. USA 1970, 65:129-136
IZSBS catalog code: BS TCL 128
Continuous culture, grown as monolayer
Neuron-like morphology
SPECIES: mouse, albino Karyology: 2n = 40 in 53 cells
TUMOR: neuroblastoma
tumorigenic in albino mice strain
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
DEPOSITOR: obtained from ATCC
PROPERTIES: used for studies on the mechanism of vinblastine precipitation of microtubular protein, the kinetics of GTP binding to single protein, the synthesis and assembly of microtubular protein
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL-131, DSMZ: ACC 148, ICLC: ATL99007
Bibliographic references:
- J Cell Biol 1969;43:69a
- Proc Natl Acad Sci U S A 1970;65:129-136 - PMID: 5263744
Continuous culture, grown as monolayer
neuron-like and amoeboid-like morphology
SPECIES: mouse, albino
TUMOR: neuroblastoma
tumorigenic in syngeneic animals
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
PROPERTIES: microtubular protein prod.; tumorigenicity
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 131, DSMZ: ACC 148, ICLC: ATL99007, IZSBS: BS TCL 128
Bibliographic references:
- J Cell Biol 1969;43:69a
- Proc Natl Acad Sci U S A 1970;65:129-136 - PMID: 5263744
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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