NCTC clone 929's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

NCTC clone 929 (mouse, C3H/An, connective)

SPECIES: mouse, C3H/An
TISSUE/ORGAN: connective

ECACC catalog code: 88102702
Morphology: Fibroblast
Susceptibility to viruses: paramyxovirus * pseudorabies * vesic. stomatitis (Indiana)
Description: Mouse C3H/An connective tissue
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Replication, biochemical, neoplastic transformation, nutritional, metabolic enzyme, morphological, chromosome, immunologic, radiation, toxicologic and virus studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Freeze in 9% glycerol/91% FBS.
Passages: 565; Karyotype: 2n = 40
From parental strain L which was derived from normal subcutaneous areolar and adipose tissue of 100 day old male C3H/An. Used in development of replicate culture techniques, chemically defined media, fluid suspension culture methods and virus assays. Susceptible to pseudorabies virus and vesicular stomatitis (Indiana strain) virus.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 1

Bibliographic references:

J Natl Cancer Inst 1948;9:229
J Natl Cancer Inst 1951;12:133
Natl Cancer Inst Monogr 1962;7:147

NCTC clone 929 (mouse, C3H/An, connective)

Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: mouse, C3H/An (male)
TISSUE/ORGAN: connective
Susceptibility to viruses: paramyxovirus * pseudorabies * vesic. stomatitis (Indiana)
PROPERTIES: tumor necrosis factor assay; biochemistry; virology; replication

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)
mycoplasma negative

Availability in cell line catalogues: ATCC: CCL 1, ECACC: 88102702

Bibliographic references:

J Natl Cancer Inst 1948;9:229
J Natl Cancer Inst 1951;12:133
Natl Cancer Inst Monogr 1962;7:147

NCTC clone 929 (mouse, C3H/An, connective)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CCL 1, ECACC: 88102702

Bibliographic references:

J Natl Cancer Inst 1948;9:229
J Natl Cancer Inst 1951;12:133
Natl Cancer Inst Monogr 1962;7:147

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105