B95.8's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

B95.8 (monkey, marmoset, lymphocyte, B)

ICLC catalog code: ATL95004
Continuous culture, grown as semiadherent
lymphoblast morphology
SPECIES: monkey, marmoset
TISSUE/ORGAN: lymphocyte, B
TRANSFORMED BY: 17
Endogenous viruses: contains Epstein-Barr
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution, only as frozen ampoule. For non-commercial investigative use only
PROPERTIES: virus production; EBV transformation

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split 1:5-1:10. Maintain cultures between 3-9 x 105 cells/ml. 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Handle under Biosafety Level 2 containment

Bibliographic references:

Experientia 1996;52:818-826 - PMID: 8774755
Proc Natl Acad Sci USA 1972;69:383-387 - PMID: 4333982
Proc Natl Acad Sci USA 1973;70:190-194 - PMID: 4346033

B95.8 (monkey, marmoset, lymphocyte, B)

Continuous culture, grown as suspension
lymphoblast and fibroblast morphology
SPECIES: monkey, marmoset
TISSUE/ORGAN: lymphocyte, B
TRANSFORMED BY: 17
Endogenous viruses: contains Epstein-Barr
PROPERTIES: virus prod.; virology; transformation

Available in the following LABORATORY:

Istituto Genetica Biochimica ed Evoluzionistica, CNR (PVCIM, Pavia)
RPMI Duch modification + 10% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative

Availability in cell line catalogues: ATCC: CRL 1612, ICLC: ATL95004

Bibliographic references:

Proc Natl Acad Sci U S A 1973;70:190-194 - PMID: 4346033

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105