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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Namalwa (human, African, lymphoma, Burkitt)

Available in the following LABORATORY:

• German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
  90% RPMI 1640 + 10% FBS maintain at 0.5-1.5 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 maximum density at about 2.0 x 106 cells/ml or higher; doubling time of about 20-30 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
  Validated by: cytogenetics 1 (hypodiploid with 4% polyploidy, a supernumerary ring or fragment present in 8% of cells; 44(43-46)<2n>X, -Y -5, -6, +7, -10, -13, -21, +3m, ins(1;?)(p11;?), ins(3)(q11q29), der(5)t(1;5)(q25;q35), del(8)(p22), der(14)t(14;?) * cytogenetics 2 (der(15)t(15;21)(p11;q11); NAMALWA marker chromosomes present) * DNA fingerprinting (HinfI-(gtg)5 DNA profile=NAMALWA subclones, otherwise unique) * immunological analysis (CD3- CD13- CD19+ CD37+ DR+ cy/sIgM+) * isoenzymes (confirmed as human with IEF of AST, NP)
  

Availability in cell line catalogues: ATCC: CRL 1432, IZSBS: BS TCL 47, ECACC: 87060801

Bibliographic references:

• Antimicrob Agents Chemother 1979;15:420 - PMID: 465469
  
• Cancer 1969;23:64-79 - PMID: 4178827
  
• Dev Biol Stand 1978;38:343
  
• Int J Cancer 1972;10:44 - PMID: 4122458
  
• Int J Cancer 1973;12:396 - PMID: 4365093
  
• J Gen Virol 1982;63:207 - PMID: 7175502
  

Namalwa (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

• Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
  RPMI 1640 + 10% FBS or MEM (EBSS) + 20% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
  Patent strain, distribution is subjected to authorization by the assignee.
  Validated by: isoenzymes (confirmed as human with NP, G6PD, MD, PEPB, AST)
  

Availability in cell line catalogues: ATCC: CRL 1432, ECACC: 87060801, DSMZ: ACC 24

Bibliographic references:

• Dev Biol Stand 1978;38:343
• Int J Cancer 1973;12:396 - PMID: 4365093
• J Gen Virol 1982;63:207 - PMID: 7175502

Namalwa (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

• Russian Academy of Medical Sciences (MWIIW, Moscow)
  mycoplasma negative
  

Availability in cell line catalogues: ATCC: CRL 1432, IZSBS: BS TCL 47, ECACC: 87060801, DSMZ: ACC 24

Bibliographic references:

• Antimicrob Agents Chemother 1979;15:420 - PMID: 465469
• Cancer 1969;23:64-79 - PMID: 4178827
• Dev Biol Stand 1978;38:343
• Int J Cancer 1972;10:44 - PMID: 4122458
• Int J Cancer 1973;12:396 - PMID: 4365093
• J Gen Virol 1982;63:207 - PMID: 7175502

Namalwa (human, Black, lymphoma, Burkitt)

Available in the following LABORATORY:

• Russian Academy of Sciences (SPBIC, St. Petersburg)
  
  

Availability in cell line catalogues: ATCC: CRL 1432, IZSBS: BS TCL 47, ECACC: 87060801, DSMZ: ACC 24

Bibliographic references:

• Antimicrob Agents Chemother 1979;15:420 - PMID: 465469
• Cancer 1969;23:64-79 - PMID: 4178827
• Dev Biol Stand 1978;38:343
• Int J Cancer 1972;10:44 - PMID: 4122458
• Int J Cancer 1973;12:396 - PMID: 4365093
• J Gen Virol 1982;63:207 - PMID: 7175502