NAMALWA's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

NAMALWA (human, African, lymphoma, Burkitt)

SPECIES: human, African
TUMOR: lymphoma, Burkitt

DSMZ catalog code: ACC 24
Morphology: lymphoblastoid, single cells or small clusters in suspension
Description: human Burkitt's lymphoma established from the tumor mass of an African child with Burkitt's lymphoma in 1967; cells express c-myc mRNA confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.5 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 maximum density at about 2.0 x 106 cells/ml or higher; doubling time of about 20-30 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypodiploid with 4% polyploidy, a supernumerary ring or fragment present in 8% of cells; 44(43-46)<2n>X, -Y -5, -6, +7, -10, -13, -21, +3m, ins(1;?)(p11;?), ins(3)(q11q29), der(5)t(1;5)(q25;q35), del(8)(p22), der(14)t(14;?) * cytogenetics 2 (der(15)t(15;21)(p11;q11); NAMALWA marker chromosomes present) * DNA fingerprinting (HinfI-(gtg)5 DNA profile=NAMALWA subclones, otherwise unique) * immunological analysis (CD3- CD13- CD19+ CD37+ DR+ cy/sIgM+) * isoenzymes (confirmed as human with IEF of AST, NP)

Availability in cell line catalogues: ATCC: CRL 1432, IZSBS: BS TCL 47, ECACC: 87060801

Bibliographic references:

Antimicrob Agents Chemother 1979;15:420 - PMID: 465469
Cancer 1969;23:64-79 - PMID: 4178827
Dev Biol Stand 1978;38:343
Int J Cancer 1972;10:44 - PMID: 4122458
Int J Cancer 1973;12:396 - PMID: 4365093
J Gen Virol 1982;63:207 - PMID: 7175502

NAMALWA (human, blood, lymphoma, Burkitt)

SPECIES: human
TISSUE/ORGAN: blood
TUMOR: lymphoma, Burkitt

ECACC catalog code: 87060801
Morphology: Lymphoblast
Description: Human Burkitt's Lymphoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe patent claims.
Properties: Products: Immunoglobulin M (IgM), lambda Applications: Interferon studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C. A maximum density of 2x1,000,000 cells/ml is possible.
Karyotype: Modal No. 44; hypodiploid
Secretes small amounts of an IgM monoclonal antibody of unknown specificity. It has been used for commercial production of human interferon. The cells contain the EBV genome and should be handled under laboratory containment level 2.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1432, IZSBS: BS TCL 47, DSMZ: ACC 24

Bibliographic references:

Antimicrob Agents Chemother 1979;15:420 - PMID: 465469
Cancer 1969;23:64-79 - PMID: 4178827
Dev Biol Stand 1978;38:343
Int J Cancer 1972;10:44 - PMID: 4122458
Int J Cancer 1973;12:396 - PMID: 4365093
J Gen Virol 1982;63:207 - PMID: 7175502

NAMALWA (human, Black, lymphoma, Burkitt)

IZSBS catalog code: BS TCL 47
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (child) Karyology: unknown
TUMOR: lymphoma, Burkitt
DEPOSITOR: obtained from ATCC
PROPERTIES: interferon alpha prod.; virology

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
RPMI 1640 + 10% FBS or MEM (EBSS) + 20% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Patent strain, distribution is subjected to authorization by the assignee.
Validated by: isoenzymes (confirmed as human with NP, G6PD, MD, PEPB, AST)

Availability in cell line catalogues: ATCC: CRL 1432, ECACC: 87060801, DSMZ: ACC 24

Bibliographic references:

Dev Biol Stand 1978;38:343
Int J Cancer 1973;12:396 - PMID: 4365093
J Gen Virol 1982;63:207 - PMID: 7175502

NAMALWA (human, Black, lymphoma, Burkitt)

Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Black (child)
TUMOR: lymphoma, Burkitt
PROPERTIES: IgM prod.; interferon alpha prod.; interferon; c-myc mRNA expression; virology

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CRL 1432, IZSBS: BS TCL 47, ECACC: 87060801, DSMZ: ACC 24

Bibliographic references:

Antimicrob Agents Chemother 1979;15:420 - PMID: 465469
Cancer 1969;23:64-79 - PMID: 4178827
Dev Biol Stand 1978;38:343
Int J Cancer 1972;10:44 - PMID: 4122458
Int J Cancer 1973;12:396 - PMID: 4365093
J Gen Virol 1982;63:207 - PMID: 7175502

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105