N1E-115's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

N1E-115 (mouse, A/Jax, nervous system, neuroblastoma)

SPECIES: mouse, A/Jax
TISSUE/ORGAN: nervous system
TUMOR: neuroblastoma

ECACC catalog code: 88112303
Morphology: Neuronal
Description: Mouse neuroblastoma
Viruses: See Properties
Depositor: Dr Nirenberg, Laboratory of Biochemical Genetics, National Institute of Health, Bethesda, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 30; Adrenergic clone derived from the mouse neuroblastoma C1300 tumour.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 2263

Bibliographic references:

Proc Natl Acad Sci USA 1972;69:258-263 - PMID: 4400294

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105