Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
Cell line available under some conditions.
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362
Bibliographic references:
- Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
- J Immunol 1982;129:2504-2510 - PMID: 6982930
- J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231
SPECIES: human
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
ECACC catalog code: 85011413
Morphology: Suspension
Susceptibility to viruses: alpha viruses * measles
Description: Human acute T lymphoblastic leukaemia
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies
Available in the following LABORATORY:
-
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Karyotype: Modal No. 95; hypertetraploid
A suspension culture derived from the peripheral blood of a 19 year old male with acute lymphoblastic leukaemia in relapse. A stable T-cell leukaemia that forms rosettes with sheep erythrocytes.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, IZSBS: BS TCL 85, DSMZ: ACC 362
Bibliographic references:
- Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
- J Immunol 1982;129:2504-2510 - PMID: 6982930
- J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231
Monoclonal continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362
Bibliographic references:
- Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
- J Immunol 1982;129:2504-2510 - PMID: 6982930
- J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231
ICLC catalog code: HTL99025
Continuous culture, grown as suspension
round cells, single or in clumps, lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, T cell
DEPOSITOR: Dr G. Canti, Pharmacology Dept, Milan, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: G->A mutation at codon 248 of the p53; CD1+ CD2+ CD3+ CD4+ CD5+ CD6+ CD7+ CD19- EBV-; high level of terminal deoxynucleotidyl transferase
Available in the following LABORATORY:
-
IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 20% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 5-10 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362
Bibliographic references:
- J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231
- J Natl Cancer Inst 1980;65;715:718 - PMID: 6932523
- Proc Natl Acad Sci USA 1990;87:7555-7559 - PMID: 16992228
IZSBS catalog code: BS TCL 85
Continuous culture, grown as suspension
Lymphoblast-like morphology
SPECIES: human
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia
DEPOSITOR: obtained from ATCC
Distribution is subjected to authorization by the assignee.
Patent strain
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL-1582, ICLC: HTL99025, ECACC: 85011413, DSMZ: ACC 362
Bibliographic references:
- J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362
Bibliographic references:
- Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
- J Immunol 1982;129:2504-2510 - PMID: 6982930
- J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231
Monoclonal continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362
Bibliographic references:
- Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
- J Immunol 1982;129:2504-2510 - PMID: 6982930
- J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362
Bibliographic references:
- Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
- J Immunol 1982;129:2504-2510 - PMID: 6982930
- J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362
Bibliographic references:
- Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
- J Immunol 1982;129:2504-2510 - PMID: 6982930
- J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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