B3/AN's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

B3/AN (mouse, AKR, thymus, thymoma)

ICLC catalog code: ATL97004
Continuous culture, grown as suspension
spherical morphology
SPECIES: mouse, AKR
TISSUE/ORGAN: thymus
TUMOR: thymoma
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: Lyt-2/13 negative cell line, studies of the assembly and transport of Lyt-2/13 antigen in the cell surface and definition of the regions of Lyt-2/13 interacting with corresponding antibodies and with MHC Class I molecules

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% HS + 2mM L-Glutamine + 1mM Na pyruvate; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Maintain cultures between 3 and 9x105 cells/ml. 37C, 5-10% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with MD, NP)

Availability in cell line catalogues: ECACC: 94031009

Bibliographic references:

Immunogenetics 1994;40:154 - PMID: 7517914
J Immunol Methods 1992;154:211 - PMID: 1401954

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105