SPECIES: rat, buffalo
TISSUE/ORGAN: liver
TUMOR: hepatoma
DSMZ catalog code: ACC 106
Morphology: adherent cells growing in big clusters, different cellular forms
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
Description: rat hepatoma a clonal strain of epithelial cells was derived from a transplantable Morris hepatoma ( 7795, Buffalo strain) in 1967; cells were described to synthesize and to secrete serum albumin and serum complement component C9 confirmed as rat with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. M. Ferro, Institute of General Pathology, University of Genova, Genova, Italy
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85-90% Dulbecco's MEM + 10-15% FBS split confluent culture 1:2 to 1:4 about once or twice per week using trypsin/EDTA; seed out at ca. 2 x 106 cells/75 cm 2 at 37C with 5-10% CO2 cell harvest of about 20 x 106 cells/175 cm 2;
doubling time of ca. 33-60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-diploid rat karyotype with 10% polyploidy; 44(40-46)<2n>) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as rat with IEF of MPI, NP) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CCL 144, ECACC: 85112702, ICLC: ATL98014
Bibliographic references:
- Cell Biochem Funct 1988;6:245-250 - PMID: 3191583
- Chem Biol Interact 1988;65:629-634
- Exp Cell Res 1991;196:330-336 - PMID: 1716586
- In Vitro 1970;6:32 - PMID: 4399803
- J Cell Biol 1969;40:236-247 - PMID: 4177659
- J Cell Res 1969;40:236
- Science 1970;170:533 - PMID: 4908238
Monoclonal continuous culture, grown as monolayer
epithelial morphology
SPECIES: rat, buffalo (male) Karyology: chromosome fragments and breaks
TISSUE/ORGAN: liver
TUMOR: hepatoma
tumorigenic in syngeneic animals
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
Cell line available under some conditions.
PROPERTIES: serum albumin prod.; serum complement components prod.; monooxygenases expression; tyrosine aminotransferase synthesis; enzymology; metabolism; toxicity screening; cytotoxicity; xenobiotic metabolism; hormone studies
Available in the following LABORATORY:
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Istituto Patologia Generale, Universita' degli Studi (GEUCC, Genova)
Ham's F10 + 7.5% NBCS + 10 HS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-5 Passage: 70 , mycoplasma negative, HOECHST
Chromosome markers: 1 large chromosome with submedian centromere; 1-2 large chromosomes with median centromeres. Derived from hepatoma induced by N-2-fluorenylpthalamic acid.
Validated by: monooxygenase expression and induction
Availability in cell line catalogues: ATCC: CCL 144, ECACC: 85112702, DSMZ: ACC 106, ICLC: ATL98014
Bibliographic references:
- Cell Biochem Funct 1988;6:245-250 - PMID: 3191583
- Chem Biol Interact 1988;65:629-634
- Exp Cell Res 1991;196:330-336 - PMID: 1716586
- In Vitro 1970;6:32 - PMID: 4399803
- J Cell Biol 1969;40:236-247 - PMID: 4177659
- J Cell Res 1969;40:236
- Science 1970;170:533 - PMID: 4908238
ICLC catalog code: ATL98014
Continuous culture, grown as monolayer
epithelial; the cells grow in large clusters morphology
SPECIES: rat
TISSUE/ORGAN: liver
TUMOR: hepatoma
DEPOSITOR: Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells synthesize and secrete serum albumin, C9, tyrosine aminotransferase; monooxigenases expression; toxicity screening; xenobiotic metabolism; drug metabolism assays; hepatocyte studies
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-2 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
cell harvest is about 10 x 10^6 cells/25 cm2 flask
Validated by: isoenzymes (confirmed as rat with AST, MD)
Availability in cell line catalogues: ECACC: 85112702, DSMZ: ACC 106
Bibliographic references:
- Cell Biochem Funct 1988;6:245-250 - PMID: 3191583
- Exp Cell Res 1991;196:330-336 - PMID: 1716586
- J Cell Biol 1969;40:236-247 - PMID: 4177659
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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